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The STa\u2010related decapeptide used, which was encoded by a synthetic oligodeoxynucleotide, contained a single mutation which substituted a disulfide\u2010linked cysteine by alanine. After its fusion to CTB the decapeptide was able to both react with and to give rise to anti\u2010STa antibodies. Expression of the decapeptide\u2010CTB hybrid by non\u2010toxigenic <jats:italic>Vibrio cholerae<\/jats:italic> resulted in its full secretion into the extracellular milieu from where it could then be readily purified by single\u2010step affinity chromatography using immobilized GM1 ganglioside. Bacteria producing this non\u2010toxic, immunogenic decapeptide\u2010CTB toxoid might be useful for the development of oral vaccines against diarrhea caused by <jats:italic>E.coli<\/jats:italic> and other bacteria producing immunologically related heat\u2010stable enterotoxins, and as a source of immunoreagents for methods used to diagnose disease caused by these bacteria.<\/jats:p>","DOI":"10.1016\/0014-5793(88)81041-x","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:45:33Z","timestamp":1027583133000},"page":"110-114","source":"Crossref","is-referenced-by-count":70,"title":["Genetic fusion of a non\u2010toxic heat\u2010stable enterotoxin\u2010related decapeptide antigen to cholera toxin B\u2010subunit"],"prefix":"10.1002","volume":"241","author":[{"given":"J.","family":"Sanchez","sequence":"first","affiliation":[]},{"given":"A.-M.","family":"Svennerholm","sequence":"additional","affiliation":[]},{"given":"J.","family":"Holmgren","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,10,29]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"23","volume-title":"11th Nobel Conf., Stockholm 1985","author":"Black R.E.","year":"1986"},{"key":"e_1_2_1_3_1","first-page":"477","volume-title":"Acute Enteric Infections in Children: New Prospects for Treatment and Prevention","author":"Moon H.W.","year":"1986"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.21.2.526-531.1978"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(83)80469-4"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1982.tb07047.x"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.56.8.2144-2148.1988"},{"key":"e_1_2_1_8_1","author":"Svennerholm A.-M.","year":"1988","journal-title":"FEMS Microbiol. 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