{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T15:27:52Z","timestamp":1773674872531,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2001,10,23]],"date-time":"2001-10-23T00:00:00Z","timestamp":1003795200000},"content-version":"vor","delay-in-days":4607,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1989,3,13]]},"abstract":"<jats:p>The isoquinoline carboxamide derivative [<jats:sup>3<\/jats:sup>H]PK11195, a ligand for the peripheral\u2010type benzodiazepine (BZD) receptor, binds to Chinese hamster ovary (CHO) cell mitochondria in a specific and saturable manner. Scatchard analysis showed the presence of a single\u2010binding site with an apparent dissociation constant (<jats:italic>K<\/jats:italic>\n<jats:sub>d<\/jats:sub>) of 12.0 \u00b1 1.0 nM and a maximal binding capacity of 23.0 \u00b1 2.0 pmol\/mg protein. The pharmacological characterization of this CHO BZD\u2010binding site, based on the displacement of [<jats:sup>3<\/jats:sup>H]PK11195 by several drugs of known binding specificity, indicated that it is of the peripheral\u2010type. The photoaffinity probe [<jats:sup>3<\/jats:sup>H]PK14105, a nitrophenyl derivative of [<jats:sup>3<\/jats:sup>H]PK11195, specifically labeled a 17 kDa CHO mitochondrial protein. This 17 kDa protein was purified from digitonin\u2010solubilized mitochondria by gel\u2010filtration chromatography and two reverse\u2010phase HPLC steps. The purified material migrated as a single band on silver stained or autoradiographed SDS\u2010polyacrylamide gels, and had an amino acid composition corresponding to a 17 kDa protein rich in Leu, Val, Ala, Gly, and Pro. Analysis of the amino\u2010terminal sequence of the purified 17 kDa protein revealed a blocked amino\u2010terminus.<\/jats:p>","DOI":"10.1016\/0014-5793(89)80229-7","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:45:33Z","timestamp":1027583133000},"page":"238-244","source":"Crossref","is-referenced-by-count":42,"title":["Characterization of a peripheral\u2010type benzodiazepine\u2010binding site in the mitochondria of Chinese hamster ovary cells"],"prefix":"10.1002","volume":"245","author":[{"given":"J.","family":"Riond","sequence":"first","affiliation":[]},{"given":"N.","family":"Vita","sequence":"additional","affiliation":[]},{"given":"G.","family":"Le Fur","sequence":"additional","affiliation":[]},{"given":"P.","family":"Ferrara","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,10,23]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.9.3805"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(81)90405-2"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(82)90164-2"},{"key":"e_1_2_1_5_1","first-page":"38","volume":"266","author":"Benavides J.","year":"1983","journal-title":"Arch. 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