{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T13:41:00Z","timestamp":1775914860753,"version":"3.50.1"},"reference-count":13,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,10,19]],"date-time":"2001-10-19T00:00:00Z","timestamp":1003449600000},"content-version":"vor","delay-in-days":4561,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1989,4,24]]},"abstract":"<jats:p>We developed specific, C\u2010terminal radioimmunoassays for three proglucagon (PG) fragments: PG 151\u2013158, PG 151\u2013160 and PG 126\u2013159 (glucagon\u2010like peptide\u20102 (GLP\u20102)) in order to determine the exact C\u2010terminal sequence of the newly isolated GLP\u20102 in man and pig. The antigens and the antisera showed no mutual cross\u2010reactivity. By gel filtration of extracts of pig and human small intestine, the immunoreactivity eluting at the position of GLP\u20102 was identified by the radioimmunoassays for glucagon\u2010like peptide\u20102 (PG 126\u2013159) and for PG 151\u2013158, whereas the assay for PG 151\u2013160 was completely negative. We conclude that the C\u2010terminal amino acid residue of pig and human ileal GLP\u20102 is PG 158. Thus the basic residues, PG 159 and 160 are removed during its processing in the small intestine.<\/jats:p>","DOI":"10.1016\/0014-5793(89)81332-8","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:45:33Z","timestamp":1027583133000},"page":"193-196","source":"Crossref","is-referenced-by-count":18,"title":["Carboxypeptidase\u2010B\u2010like processing of the C\u2010terminus of glucagon\u2010like peptide\u20102 in pig and human small intestine"],"prefix":"10.1002","volume":"247","author":[{"given":"C.","family":"\u00d8rskov","sequence":"first","affiliation":[]},{"given":"T.","family":"Buhl","sequence":"additional","affiliation":[]},{"given":"L.","family":"Rabenh\u00f8j","sequence":"additional","affiliation":[]},{"given":"H.","family":"Kofod","sequence":"additional","affiliation":[]},{"given":"J.J.","family":"Holst","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,10,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/304368a0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)89859-3"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00274797"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-119-4-1467"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)68350-4"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00359a014"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00571a022"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365518709168885"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1530\/acta.0.0980564"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.18.5485"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/302716a0"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.2.345"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(84)81245-4"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793%2889%2981332-8","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793(89)81332-8","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/0014-5793%2889%2981332-8","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T07:59:55Z","timestamp":1694851195000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/0014-5793%2889%2981332-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,4,24]]},"references-count":13,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1989,4,24]]}},"alternative-id":["10.1016\/0014-5793(89)81332-8"],"URL":"https:\/\/doi.org\/10.1016\/0014-5793(89)81332-8","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,4,24]]}}}