{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T05:57:40Z","timestamp":1779343060152,"version":"3.51.4"},"reference-count":17,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2001,10,29]],"date-time":"2001-10-29T00:00:00Z","timestamp":1004313600000},"content-version":"vor","delay-in-days":4060,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1990,9,17]]},"abstract":"<jats:p>cDNA encoding the putative \u03b1\u2010latrotoxin precursor was isolated from spider venom glands cDNA library and sequenced. The cDNA contained the 4203 base\u2010pair open reading frame corresponding to the 156 855\u2010Da protein composed of 1401 amino acids. Computer analysis of the deduced primary structure revealed the presence of various internal imperfect repeats mainly in its central and C\u2010tenninal regions.<\/jats:p>","DOI":"10.1016\/0014-5793(90)81250-r","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T09:10:37Z","timestamp":1027588237000},"page":"127-131","source":"Crossref","is-referenced-by-count":96,"title":["Cloning and structure of cDNA encoding \u03b1\u2010latrotoxin from black widow spider venom"],"prefix":"10.1002","volume":"270","author":[{"given":"N.I.","family":"Kiyatkin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.E.","family":"Dulubova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.A.","family":"Chekhovskaya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.V.","family":"Grishin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,10,29]]},"reference":[{"key":"e_1_2_1_2_1","unstructured":"1988 Ellis Horwood A. Grasso J.O. Dolly Neurotoxins in neurochemistry 67 78"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1985.tb03632.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.948756"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(87)90345-2"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)33927-9"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.6.1408"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.6.2726"},{"key":"e_1_2_1_9_1","author":"Volkova T.M.","year":"1990","journal-title":"Bioorg. Khim."},{"key":"e_1_2_1_10_1","unstructured":"1982 Cold Spring Harbor Laboratory Cold Spring Harbor New York T. Maniatis E.F. Fritsch J. Sambrook Molecular Cloning: a Laboratory Manual"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(85)80185-X"},{"key":"e_1_2_1_12_1","first-page":"515","volume":"14","author":"Kostetsky P.V.","year":"1988","journal-title":"Bioorg. Khim."},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00405a050"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.18.9048"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(85)80046-8"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(86)90293-2"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90229-6"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-69-5-1025"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793%2890%2981250-R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793(90)81250-R","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/0014-5793%2890%2981250-R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T15:53:56Z","timestamp":1694879636000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/0014-5793%2890%2981250-R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,9,17]]},"references-count":17,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[1990,9,17]]}},"alternative-id":["10.1016\/0014-5793(90)81250-R"],"URL":"https:\/\/doi.org\/10.1016\/0014-5793(90)81250-r","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,9,17]]}}}