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The phosphopeptides were isolated by high performance liquid chromatography and sequenced. A comparison of the primary structure of the phosphopeptides with that of EF\u20102 showed that all 4 phosphopeptides originated from one region of EF\u20102 located near the N\u2010terminus that contains 3 threonine residues: Thr\u201053, Thr\u201056, Thr\u201058. A direct estimation of localization of radioactive phosphate in the phosphopeptides demonstrated that all the enumerated threonine residues in EF\u20102 can be phosphorylated in vitro.<\/jats:p>","DOI":"10.1016\/0014-5793(90)81473-2","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:44:17Z","timestamp":1027583057000},"page":"209-212","source":"Crossref","is-referenced-by-count":49,"title":["Three phosphorylation sites in elongation factor 2"],"prefix":"10.1002","volume":"275","author":[{"given":"Lev P.","family":"Ovchinnikov","sequence":"first","affiliation":[]},{"given":"Lyudmila P.","family":"Motuz","sequence":"additional","affiliation":[]},{"given":"Pavel G.","family":"Natapov","sequence":"additional","affiliation":[]},{"given":"Lidiya J.","family":"Averbuch","sequence":"additional","affiliation":[]},{"given":"Richard E.H.","family":"Wettenhall","sequence":"additional","affiliation":[]},{"given":"Ryszard","family":"Szyszka","sequence":"additional","affiliation":[]},{"given":"Gisela","family":"Kramer","sequence":"additional","affiliation":[]},{"given":"Boyd","family":"Hardesty","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,12,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)91956-3"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.45.070176.001203"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a131774"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(87)80081-9"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.23.7939"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)45377-X"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0300-9084(88)90245-3"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/334170a0"},{"key":"e_1_2_1_10_1","first-page":"1495","volume":"297","author":"Shestakova E.A.","year":"1987","journal-title":"Dokl. 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