{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T20:32:08Z","timestamp":1718656328396},"reference-count":18,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2002,1,9]],"date-time":"2002-01-09T00:00:00Z","timestamp":1010534400000},"content-version":"vor","delay-in-days":4223,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1990,6,18]]},"abstract":"<jats:p>The patch\u2010clamp technique in the cell\u2010attached mode was used to study the K channels present in the membrane of cultured pancreatic \u03b2 cells from <jats:italic>ob<\/jats:italic>\/<jats:italic>ob<\/jats:italic> mice. Three types of K<jats:sup>+<\/jats:sup> channels were regularly observed, with conductances of 64, 20 and 146 pS. The conduction and kinetic properties of the 64 pS channel were similar to those of the ATP\u2010sensitive potassium channel from normal \u03b2 cells. Furthermore, glucose blocked the activity of this channel at the same concentrations as that reported for normal cells. The 20 pS and the 146 pS were insensitive to glucose. The latter K<jats:sup>+<\/jats:sup> channel appears to be similar to the large conductance voltage\u2010activated potassium channels described in normal rodent \u03b2 cells. Thus, potassium channels in <jats:italic>ob<\/jats:italic>\/<jats:italic>ob<\/jats:italic> pancreatic \u03b2 cells in culture are in most respects normal. Other factors may account for the abnormal electrical response to glucose of <jats:italic>ob<\/jats:italic>\/<jats:italic>ob<\/jats:italic> pancreatic islets, such as reversible impairment of their function in vivo or defects not related to potassium permeability.<\/jats:p>","DOI":"10.1016\/0014-5793(90)81518-s","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:44:17Z","timestamp":1027583057000},"page":"105-108","source":"Crossref","is-referenced-by-count":5,"title":["Characterization of potassium channels in pancreatic \u03b2 cells from <i>ob<\/i>\/<i>ob<\/i> mice"],"prefix":"10.1002","volume":"266","author":[{"given":"Manuel","family":"Kukuljan","sequence":"first","affiliation":[]},{"given":"Min Yi","family":"Li","sequence":"additional","affiliation":[]},{"given":"Illani","family":"Atwater","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2002,1,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1978.sp012296"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01971450"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1979.tb06431.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(76)80567-4"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.1985.sp002885"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-5314-0_9"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(83)98149-1"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00656997"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/312446a0"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/311271a0"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.38.4.422"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01871181"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1988.sp017134"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/311269a0"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1986.sp016096"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00585619"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112389"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.37.4.398"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793%2890%2981518-S","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793(90)81518-S","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/0014-5793%2890%2981518-S","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T03:44:50Z","timestamp":1694835890000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/0014-5793%2890%2981518-S"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6,18]]},"references-count":18,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[1990,6,18]]}},"alternative-id":["10.1016\/0014-5793(90)81518-S"],"URL":"https:\/\/doi.org\/10.1016\/0014-5793(90)81518-s","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6,18]]}}}