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The cloned cDNA encodes a polypeptide composed of 257 amino acid residues including a prepro\u2010sequence of 138 residues and a mature region of 119 residues. The amino acid sequence of human NGF\u20102 exhibits 58% similarity with that of human NGF. Conditioned medium of COS\u20107 cells transfected with an expression plasmid for human NGF\u20102 cDNA supported the survival of sensory neurons isolated from dorsal root ganglia of embryonic chicks. A 1.5 kb of NGF\u20102 mRNA can be detected from an early development stage in rat brain, by Northern blotting analysis.<\/jats:p>","DOI":"10.1016\/0014-5793(90)81536-w","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T09:10:37Z","timestamp":1027588237000},"page":"187-191","source":"Crossref","is-referenced-by-count":151,"title":["Cloning and expression of a cDNA encoding a novel human neurotrophic factor"],"prefix":"10.1002","volume":"266","author":[{"given":"Yoshihiko","family":"Kaisho","sequence":"first","affiliation":[]},{"given":"Koji","family":"Yoshimura","sequence":"additional","affiliation":[]},{"given":"Kazuo","family":"Nakahama","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2002,1,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.68.10.2417"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1980.60.4.1284"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(83)90107-4"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1982.tb01207.x"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(85)90402-6"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.06-07-01897.1986"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/341149a0"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/303821a0"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00591a005"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.6.1408"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(73)90163-3"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(85)90435-X"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3576226"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/344339a0"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2321006"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.24.7951"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1984.tb02277.x"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.3.817"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793%2890%2981536-W","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793(90)81536-W","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/0014-5793%2890%2981536-W","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T03:45:50Z","timestamp":1694835950000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/0014-5793%2890%2981536-W"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6,18]]},"references-count":19,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[1990,6,18]]}},"alternative-id":["10.1016\/0014-5793(90)81536-W"],"URL":"https:\/\/doi.org\/10.1016\/0014-5793(90)81536-w","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6,18]]}}}