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Site\u2010directed mutagenesis demonstrates that Arg<jats:sup>909<\/jats:sup> and Thr<jats:sup>912<\/jats:sup> at the C\u2010terminus are important for the increase in <jats:italic>V<\/jats:italic>\n<jats:sub>max<\/jats:sub> of the ATPase induced by glucose. Other changes in kinetic parameters induced by glucose are largely independent of these ammo acids. Arg<jats:sup>909<\/jats:sup> and Thr<jats:sup>912<\/jats:sup> form a potential phosphorylation site for calmodulin\u2010dependent multiprotein kinase. A double mutation of Ser<jats:sup>911<\/jats:sup> and Thr<jats:sup>912<\/jats:sup> to Ala results in no cell growth in glucose medium and greatly reduced activation of the ATPase by glucose. Growth and activity are restored by a third mutation (Ala<jats:sup>547<\/jats:sup> \u2192 Val) at the catalytic domain, providing genetic evidence for domain interaction.<\/jats:p>","DOI":"10.1016\/0014-5793(91)80018-x","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T09:09:11Z","timestamp":1027588151000},"page":"71-74","source":"Crossref","is-referenced-by-count":91,"title":["Analysis of the regulatory domain of yeast plasma membrane H<sup>+<\/sup>\u2010ATPase by directed mutagenesis and intragenic suppression"],"prefix":"10.1002","volume":"287","author":[{"given":"Francisco","family":"Portillo","sequence":"first","affiliation":[]},{"given":"Pilar","family":"Eraso","sequence":"additional","affiliation":[]},{"given":"Ramon","family":"Serrano","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,11,16]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1989.tb15235.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(83)80237-3"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(89)81375-4"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb03010.x"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)68195-5"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00434664"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(88)57102-1"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pp.40.060189.000425"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.4.925"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)38015-3"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1988.0040"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(87)81226-7"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.21.8172"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.91.1.213"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60809-X"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.168.3.1254-1257.1986"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(89)80206-6"},{"key":"e_1_2_1_19_1","first-page":"1453","volume":"135","author":"Mazon M.J.","year":"1989","journal-title":"J. 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