{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T04:24:31Z","timestamp":1762143871798},"reference-count":35,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2001,12,17]],"date-time":"2001-12-17T00:00:00Z","timestamp":1008547200000},"content-version":"vor","delay-in-days":3738,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1991,9,23]]},"abstract":"<jats:p>Rat basophilic leukemia cells (RBL\u20102H3) have previously been shown to contain a single type of voltage\u2010activated channel, namely an inwardly rectifying K<jats:sup>+<\/jats:sup> channel, under normal recording conditions. Thus, RBL\u20102H3 cells seemed like a logical source of mRNA for the expression cloning of inwardly rectifying K<jats:sup>+<\/jats:sup> channels. Injection of mRNA isolated from RBL\u20102H3 cells into <jats:italic>Xenopus<\/jats:italic> oocytes resulted in the expression of an inward current which (1) activated at potentials negative to the K<jats:sup>+<\/jats:sup> equilibrium potential (E<jats:sub>K<\/jats:sub>), (2)decreased in slope conductance near E<jats:sub>K<\/jats:sub>, (3) was dependent on [K<jats:sup>+<\/jats:sup>]<jats:sub>o<\/jats:sub> and (4) was blocked by external Ba<jats:sup>2+<\/jats:sup> and Cs<jats:sup>+<\/jats:sup>. These properties were similar to those of the inwardly rectifying K<jats:sup>+<\/jats:sup> current recorded from RBL\u20102H3 cells using whole\u2010cell voltage clamp. Injection of size\u2010fractionated mRNA into <jats:italic>Xenopus<\/jats:italic> oocytes revealed that the current was most strongly expressed from the fraction containing mRNA of approximately 4\u20135 kb. Expression of this channel represents a starting point for the expression cloning of a novel class of K<jats:sup>+<\/jats:sup> channels.<\/jats:p>","DOI":"10.1016\/0014-5793(91)81215-t","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:43:20Z","timestamp":1027583000000},"page":"17-21","source":"Crossref","is-referenced-by-count":22,"title":["Expression of an inwardly rectifying K<sup>+<\/sup> channel from rat basophilic leukemia cell mRNA in <i>Xenopus<\/i> oocytes"],"prefix":"10.1002","volume":"290","author":[{"given":"Deborah L.","family":"Lewis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen R.","family":"Ikeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dave","family":"Aryee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolf H.","family":"Joho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,17]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"285","volume":"3","author":"Katz B.","year":"1949","journal-title":"Arch. 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