{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T17:58:04Z","timestamp":1776362284963,"version":"3.51.2"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,11,14]],"date-time":"2001-11-14T00:00:00Z","timestamp":1005696000000},"content-version":"vor","delay-in-days":2907,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1993,11,29]]},"abstract":"<jats:p>SNAP\u201025, a membrane\u2010associated protein of the nerve terminal, is specifically cleaved by botulinum neurotoxins serotypes A and E, which cause human and animal botulism by blocking neurotransmitter release at the neuromuscular junction. Here we show that these two metallo\u2010endopeptidase toxins cleave SNAP\u201025 at two distinct carboxyl\u2010terminal sites. Serotype A catalyses the hydrolysis of the Gln<jats:sup>197<\/jats:sup>\u2010Arg<jats:sup>198<\/jats:sup> peptide bond, while serotype E cleaves the Arg<jats:sup>180<\/jats:sup>\u2010Ile<jats:sup>181<\/jats:sup> peptide linkage. These results indicate that the carboxyl\u2010terminal region of SNAP\u201025 plays a crucial role in the multi\u2010protein complex that mediates vesicle docking and fusion at the nerve terminal.<\/jats:p>","DOI":"10.1016\/0014-5793(93)80448-4","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:41:36Z","timestamp":1027582896000},"page":"99-103","source":"Crossref","is-referenced-by-count":365,"title":["Botulinum neurotoxins serotypes A and E cleave SNAP\u201025 at distinct COOH\u2010terminal peptide bonds"],"prefix":"10.1002","volume":"335","author":[{"given":"Giampietro","family":"Schiavo","sequence":"first","affiliation":[]},{"given":"Annalisa","family":"Santucci","sequence":"additional","affiliation":[]},{"given":"Bibhuti R.","family":"Dasgupta","sequence":"additional","affiliation":[]},{"given":"Prashant P.","family":"Mehta","sequence":"additional","affiliation":[]},{"given":"Jaime","family":"Jontes","sequence":"additional","affiliation":[]},{"given":"Fabio","family":"Benfenati","sequence":"additional","affiliation":[]},{"given":"Michael C.","family":"Wilson","sequence":"additional","affiliation":[]},{"given":"Cesare","family":"Montecucco","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,11,14]]},"reference":[{"key":"e_1_2_1_2_1","unstructured":"1989 Academic Press New York L.L. 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