{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T15:47:11Z","timestamp":1771688831960,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2001,10,29]],"date-time":"2001-10-29T00:00:00Z","timestamp":1004313600000},"content-version":"vor","delay-in-days":2940,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1993,10,11]]},"abstract":"<jats:p>The class C \u03b2\u2010lactamase of <jats:italic>Cltrobacter freundii<\/jats:italic> GN346 is a typical cephalosporinase comprising 361 amino acids. The aspartic acid at position 217 and glutamic acid at position 219 in this \u03b2\u2010lactamase were, respectively, previously shown not to be the counterpart of Glu<jats:sup>166<\/jats:sup> (ABL166) in class A \u03b2\u2010lactamases, even though sequence alignment of class A and C enzymes strongly suggested this possibility [(1990) FEBS Lett. 264, 211\u2010214; (1990) J. Bacteriol. 172, 4348\u20104351]. We tried again to assign candidates for the counterpart of Glu<jats:sup>166<\/jats:sup> through sequence alignment based on other criteria, the glutamic acids at positions 195 and 205 in the class C \u03b2\u2010lactamase being selected. To investigate this possibility, these two glutamic acids were changed to glutamine, lysine or alanine, respectively. All the mutant enzymes showed more than 50% of the activity of the wild\u2010type enzyme, indicating that the possibility was ruled out. These results strongly suggested the possibility that the class C \u03b2\u2010lactamase lacks a functional acidic residue corresponding to Glu<jats:sup>166<\/jats:sup> in class A enzymes.<\/jats:p>","DOI":"10.1016\/0014-5793(93)80491-c","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T09:09:11Z","timestamp":1027588151000},"page":"93-98","source":"Crossref","is-referenced-by-count":9,"title":["A survey of a functional amino acid of class C\u03b2\u2010lactamase corresponding to Glu<sup>166<\/sup> of class A 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