{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T18:27:57Z","timestamp":1761762477140},"reference-count":30,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[2001,10,29]],"date-time":"2001-10-29T00:00:00Z","timestamp":1004313600000},"content-version":"vor","delay-in-days":2940,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1993,10,11]]},"abstract":"<jats:p>A microsomal endoprotease specifically cleaves isoprenylated peptides of the CAAX motif, such as <jats:italic>N<\/jats:italic>\u2010acetyl\u2010<jats:italic>S<\/jats:italic>\u2010all\u2010<jats:italic>trans<\/jats:italic>\u2010farnesyl\u2010<jats:sc>l<\/jats:sc>\u2010cysteine (AFC\u2010VIM), at the isoprenylated cysteine residue. It is shown here that endoproteolysis will also occur with peptides which are not of the CAAX type. Peptide substrates modeled after the Delta virus large antigen carboxyl\u2010terminus (CRPQ) are endoproteolytically hydrolyzed by liver microsomes. AFC\u2010RPQ is hydrolyzed with a <jats:italic>K<\/jats:italic>\n<jats:sub>M<\/jats:sub> = 12.4 \u03bcM and a <jats:italic>V<\/jats:italic>\n<jats:sub>max<\/jats:sub>= 0.27 nmolmin\/mg, and AGGC\u2010RPQ is hydrolyzed with a <jats:italic>K<\/jats:italic>\n<jats:sub>M<\/jats:sub>= 7.9 \u03bcM and a <jats:italic>V<\/jats:italic>\n<jats:sub>max<\/jats:sub> = 0.042 nmolmin\/mg. Moreover, a series of potent inhibitors of the endoproteolysis of AFC\u2010AAX\u2010containing peptides are ineffective at inhibiting the hydrolysis of AFC\u2010RPQ and AGGC\u2010RPQ, suggesting the existence of isoforms of the endoprotease.<\/jats:p>","DOI":"10.1016\/0014-5793(93)80493-e","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:41:36Z","timestamp":1027582896000},"page":"105-110","source":"Crossref","is-referenced-by-count":10,"title":["Endoproteolysis of non\u2010CAAX\u2010containing isoprenylated peptides"],"prefix":"10.1002","volume":"332","author":[{"given":"Yu-Ting","family":"Ma","sequence":"first","affiliation":[]},{"given":"Robert R.","family":"Rando","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,10,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.19.7673"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.15.5873"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.4.15.2123808"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90054-8"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)68736-8"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.110.5.1489"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1598578"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.21.8323"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2569235"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/341384a0"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)47327-4"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.14.6196"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.14.6264"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.19.7541"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90242-7"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)77164-0"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90460-G"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1991.tb07992.x"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.13.4643"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb03478.x"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.22.8960"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.8.3043"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)46215-6"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.1.286"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00139a021"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.14.6275"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.10.4613"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00162a014"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00060a033"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2840835"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793%2893%2980493-E","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793(93)80493-E","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/0014-5793%2893%2980493-E","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T00:10:48Z","timestamp":1694909448000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/0014-5793%2893%2980493-E"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,10,11]]},"references-count":30,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[1993,10,11]]}},"alternative-id":["10.1016\/0014-5793(93)80493-E"],"URL":"https:\/\/doi.org\/10.1016\/0014-5793(93)80493-e","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,10,11]]}}}