{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T07:44:56Z","timestamp":1761291896813},"reference-count":10,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,10,29]],"date-time":"2001-10-29T00:00:00Z","timestamp":1004313600000},"content-version":"vor","delay-in-days":3115,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1993,4,19]]},"abstract":"<jats:p>Staphylococcal bi\u2010component toxins, leukocidin and \u03b3\u2010hemolysin, consist of two protein components, i.e. F and S for leukocidin and H\u03b3I and H\u03b3II for \u03b3\u2010hemolysin. In this study we purified H\u03b3I and H\u03b3II to homogeneity from the culture medium of <jats:italic>Staphylococcus aureus<\/jats:italic> RIMD 310925 and compared their properties with those of F and S purified from the same source. The N\u2010terminal 59\u2010 and C\u2010terminal 2\u2010residue amino acid sequences, apparent molecular mass, and isoelectri point of purified H\u03b3I were the same as those of F. In an Ouchterlony double diffusion test a fused line without spur was formed between F and H\u03b3I using either anti\u2010F or anti\u2010H\u03b3I antibodies. A synergistic action of F and H\u03b3II caused hemolysis of human red blood cells, and H\u03b3I acted synergistically with S to exhibit leukocidin activity. We conclude that the two toxins share one protein component (F = H\u03b3I) in common and leukocidin\u2010 and \u03b3\u2010hemolysin\u2010specific activities are determined by S and H\u03b3II, respectively. It is also reported that the N\u2010terminal 58\u2010residue sequence of H\u03b3II is 72% similar to the corresponding sequence of S.<\/jats:p>","DOI":"10.1016\/0014-5793(93)80611-w","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:42:02Z","timestamp":1027582922000},"page":"15-18","source":"Crossref","is-referenced-by-count":43,"title":["The two Staphylococcal bi\u2010component toxins, leukocidin and gamma\u2010hemolysin, share one component in common"],"prefix":"10.1002","volume":"321","author":[{"given":"Yoshiyuki","family":"Kamio","sequence":"first","affiliation":[]},{"given":"Arifur","family":"Rahman","sequence":"additional","affiliation":[]},{"given":"Hirofumi","family":"Nariya","sequence":"additional","affiliation":[]},{"given":"Toshiko","family":"Ozawa","sequence":"additional","affiliation":[]},{"given":"Kazuo","family":"Izaki","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,10,29]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"281","volume-title":"The staphylococci","author":"Woodin A.M.","year":"1972"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(88)65005-1"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(05)81392-0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(92)90637-Z"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(80)90035-5"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1989.tb00235.x"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)44109-4"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(89)90364-3"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83985-6"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac60128a025"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793%2893%2980611-W","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793(93)80611-W","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/0014-5793%2893%2980611-W","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T11:28:24Z","timestamp":1694863704000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/0014-5793%2893%2980611-W"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,4,19]]},"references-count":10,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,4,19]]}},"alternative-id":["10.1016\/0014-5793(93)80611-W"],"URL":"https:\/\/doi.org\/10.1016\/0014-5793(93)80611-w","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,4,19]]}}}