{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T13:18:56Z","timestamp":1775395136991,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,11,14]],"date-time":"2001-11-14T00:00:00Z","timestamp":1005696000000},"content-version":"vor","delay-in-days":3229,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1993,1,11]]},"abstract":"<jats:p>The cyclisation of lycopene to \u03b2\u2010carotene and the hydroxylation of \u03b2\u2010carotene to zeaxanthin are common enzymatic steps in the biosynthesis of carotenoids in a wide range of bacteria, fungi, and plants. We have individually expressed in <jats:italic>E. coli<\/jats:italic> the two genes coding for these enzymatic steps in <jats:italic>Erwinia herbicola<\/jats:italic>. The cyclase and hydroxylase enzymes have apparent molecular weights of 43 kDa and 22 kDa, respectively, as determined by SDS\u2010PAGE. Hydroxylase in vitro activity was obtained only in the cytoplasmic fraction. Cyclase also demonstrated enzyme activity in a crude cell\u2010free lysate, although to a lesser extent.<\/jats:p>","DOI":"10.1016\/0014-5793(93)81188-6","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:42:02Z","timestamp":1027582922000},"page":"329-334","source":"Crossref","is-referenced-by-count":51,"title":["In vitro expression and activity of lycopene cyclase and \u03b2\u2010carotene hydroxylase from <i>Erwinia herbicola<\/i>"],"prefix":"10.1002","volume":"315","author":[{"given":"Bhupinder S.","family":"Hundle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"O'Brien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Beyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans","family":"Kiemig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John E.","family":"Hearst","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,11,14]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-0348-5065-0"},{"key":"e_1_2_1_3_1","volume-title":"The Biochemistry of Natural Pigments","author":"Britton G.","year":"1983"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1985.tb09308.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.11.4936"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(62)90350-8"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1440231"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(85)90051-0"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1991.tb01989.x"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.168.2.607-612.1986"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.12.6704-6712.1990"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.19.9321"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2448875"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2463672"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(90)85008-C"},{"key":"e_1_2_1_16_1","first-page":"312","volume":"4","author":"Riggs M.G.","year":"1986","journal-title":"Bio-Techniques"},{"key":"e_1_2_1_17_1","volume-title":"Molecular Cloning","author":"Maniatis T.","year":"1989"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(82)80024-0"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)47341-9"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1989.tb15000.x"},{"key":"e_1_2_1_22_1","first-page":"237","volume":"6","author":"Beyer P.","year":"1989","journal-title":"Bio. Sci."}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793%2893%2981188-6","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793(93)81188-6","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/0014-5793%2893%2981188-6","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T02:51:24Z","timestamp":1694919084000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/0014-5793%2893%2981188-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,1,11]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1993,1,11]]}},"alternative-id":["10.1016\/0014-5793(93)81188-6"],"URL":"https:\/\/doi.org\/10.1016\/0014-5793(93)81188-6","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,1,11]]}}}