{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T02:00:43Z","timestamp":1760061643853},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,11,5]],"date-time":"2001-11-05T00:00:00Z","timestamp":1004918400000},"content-version":"vor","delay-in-days":3066,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1993,6,14]]},"abstract":"<jats:p>DNA fragments corresponding to the sequences of <jats:italic>Escherichia coli<\/jats:italic> tRNA<jats:sub>2<\/jats:sub>\n<jats:sup>ser<\/jats:sup> and amber suppressor tRNA<jats:sup>ser<\/jats:sup>, were synthesized from overlapping oligonucleotides. These were interposed between a strong promotor and a synthetic transcriptional terminator to ensure the production of a transcript of the correct size. The genes of promotor, fragment and terminator were cloned into a conditional runaway replication plasmid. At temperatures below 37\u00b0C this vector has a low copy number but, following a temperature shift to 42\u00b0C, the copy number is no longer regulated. Using these constructs an overexpression of tRNA<jats:sup>ser<\/jats:sup> of about 20 times the level of the wild\u2010type pool could be obtained (corresponding e.g. to 200 times the expression tRNA<jats:sub>2<\/jats:sub>\n<jats:sup>ser<\/jats:sup>). From these systems 10 mg quantities of tRNA<jats:sup>ser<\/jats:sup>s could be isolated with a serine acceptance of 1,100 pmol\/<jats:italic>A<\/jats:italic>\n<jats:sub>280<\/jats:sub> unit.<\/jats:p>","DOI":"10.1016\/0014-5793(93)81385-d","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:41:36Z","timestamp":1027582896000},"page":"162-166","source":"Crossref","is-referenced-by-count":11,"title":["In vivo overexpression and purification of <i>Escherichia coli<\/i> tRNA<sup>ser<\/sup>"],"prefix":"10.1002","volume":"324","author":[{"given":"Franck","family":"Borel","sequence":"first","affiliation":[]},{"given":"Michael","family":"H\u00e4rtlein","sequence":"additional","affiliation":[]},{"given":"Reuben","family":"Leberman","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,11,5]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2479982"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2047877"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/347203a0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.13.3489"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/347249a0"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/11.11.3823"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(85)90120-9"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.17.6548"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(84)90086-6"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/1.8.1007"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/7.6.1513"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/5.10.3665"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/8.14.3133"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.56.070187.001403"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83655-4"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83654-2"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/15.22.9109"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(93)81386-E"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/331723a0"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793%2893%2981385-D","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2F0014-5793(93)81385-D","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/0014-5793%2893%2981385-D","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T01:52:33Z","timestamp":1694915553000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/0014-5793%2893%2981385-D"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,6,14]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1993,6,14]]}},"alternative-id":["10.1016\/0014-5793(93)81385-D"],"URL":"https:\/\/doi.org\/10.1016\/0014-5793(93)81385-d","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,6,14]]}}}