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Electrophysiological studies reveal that these \u03b17\u2010nAChR function as \u03b1\u2010bungarotoxin (Bgt)\u2010sensitive, quickly activating\/inactivating ion channels with a unique pharmacological profile and an unusually high permeability to calcium ions. Although similar observations have been made in studies of Bgt\u2010sensitive, functional nAChR subtypes that are naturally expressed in neuronal cells, all attempts until now to reconstitute functional \u03b17\u2010nAChR in cell lines have failed. Here we report the successful use of SH\u2010SY5Y human neuroblastoma cells, which naturally express low levels of endogenous \u03b17 transcripts, to stably overexpress heterologous rat nAChR \u03b17 transgenes. These transgenes are expressed as the appropriately\u2010sized \u03b17 messages and protein, and stably transfected SH\u2010SY5Y cells have over 30\u2010times higher levels of specific Bgt binding sites than do wild\u2010type cells. Whole cell current recordings confirm that transfected cells express functional nAChR that are sensitive to blockade by Bgt and display the typical physiological and pharmacological profiles of \u03b17\u2010nAChR. We conclude that stable, functional expression of \u03b17 transgenes in a mammalian cell line has been achieved for the first time.<\/jats:p>","DOI":"10.1016\/0014-5793(94)01108-7","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T07:57:06Z","timestamp":1027583826000},"page":"155-159","source":"Crossref","is-referenced-by-count":96,"title":["Functional expression of nicotinic acetylcholine receptors containing rat \u03b17 subunits in human SH\u2010SY5Y neuroblastoma cells"],"prefix":"10.1002","volume":"354","author":[{"given":"Elzbieta","family":"Puchacz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Buisson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Bertrand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald J.","family":"Lukas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,10,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0033583500004352"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7742(08)60097-5"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0959-4388(93)90121-E"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(79)90335-7"},{"key":"e_1_2_1_6_1","unstructured":"1980 Spectrum Publishing New York J. 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