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Knowledge of profilin\/ligand interactions is important for understanding the physiology of profilin in the cell. As a first step in the characterization of profilin\/ligand complexes, we have studied a profilin\/poly\u2010P<jats:sc>l<\/jats:sc>\u2010prohne complex in solution using high resolution NMR spectroscopy. Analysis of profilin NOE's and chemical shift data indicates that the protein secondary structure is conserved upon binding to poly\u2010<jats:sc>l<\/jats:sc>\u2010proline and that the binding site is located between the N\u2010 and C\u2010terminal helices in a region rich in highly conserved aromatic sidechains. This site is adjacent to the proposed binding site for actin. In addition, the rate constant for dissociation of the complex is found to be 1.6 \u00b1 0.2 \u00d7 10<jats:sup>4<\/jats:sup> s<jats:sup>\u22121<\/jats:sup>.<\/jats:p>","DOI":"10.1016\/0014-5793(94)80262-9","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T09:09:11Z","timestamp":1027588151000},"page":"145-151","source":"Crossref","is-referenced-by-count":52,"title":["Elucidation of the poly\u2010<scp>l<\/scp>\u2010proline binding site in <i>Acanthamoeba<\/i> profilin I by NMR spectroscopy"],"prefix":"10.1002","volume":"337","author":[{"given":"Sharon J.","family":"Archer","sequence":"first","affiliation":[]},{"given":"Valda K.","family":"Vinson","sequence":"additional","affiliation":[]},{"given":"Thomas D.","family":"Pollard","sequence":"additional","affiliation":[]},{"given":"Dennis A.","family":"Torchia","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,10,29]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0962-8924(93)90087-H"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970200209"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(77)90166-8"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/314472a0"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240370302"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1848725"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.1.12.937"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1985.tb09099.x"},{"key":"e_1_2_1_10_1","unstructured":"1993 Johns Hopkins University L.M. 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