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In <jats:italic>E. coli<\/jats:italic>, efficient refolding of luciferase requires the Hsp70 DnaK and two modulators, DnaJ and GrpE, that synergistically stimulate its ATPase activity. Exchanging DnaJ homologs between the <jats:italic>S. cerevisiae<\/jats:italic> and <jats:italic>E. coli<\/jats:italic> systems revealed that their ability to stimulate Hsp70 ATPase activity was conserved. In contrast, GrpE further stimulated only DnaK's ATPase activity. Efficient refolding of luciferase by Ssa1p and DnaJ, but not by DnaK and Ydj1p, suggests that a compatible Hsp70\/DnaJ homolog pair can act as a protein folding machinery.<\/jats:p>","DOI":"10.1016\/0014-5793(95)00704-d","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T01:45:07Z","timestamp":1027647907000},"page":"435-440","source":"Crossref","is-referenced-by-count":61,"title":["Conserved ATPase and luciferase refolding activities between bacteria and yeast Hsp70 chaperones and modulators"],"prefix":"10.1002","volume":"368","member":"311","published-online":{"date-parts":[[2000,3,7]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"The Biology of Heat Shock Proteins and Molecular Chaperones","author":"Frydman J.","year":"1994"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/353726a0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/355455a0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90492-9"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/356683a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb06097.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2188360"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/366228a0"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90533-9"},{"key":"e_1_2_1_11_2","volume-title":"The Biology of Heat Shock Proteins and Molecular Chaperones","author":"Georgopoulos C.","year":"1994"},{"key":"e_1_2_1_12_2","first-page":"2874","volume-title":"Proc. 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