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Addition of purified DnaK and DnaJ to the system prior to the start of protein synthesis leads to the increase of the specific activity of formed CAT. A portion of exogenous DnaK and DnaJ added to the system associate with nascent polypeptide chains in the ribosomes. DnaK also comigrates with 50S\u2010ribosomal subunits.<\/jats:p>","DOI":"10.1016\/0014-5793(95)01213-x","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T01:45:07Z","timestamp":1027647907000},"page":"211-214","source":"Crossref","is-referenced-by-count":10,"title":["Synthesis of chloramphenicol acetyltransferase in a coupled transcription\u2010translation in vitro system lacking the chaperones DnaK and DnaJ"],"prefix":"10.1002","volume":"375","author":[{"given":"Alexander V","family":"Vysokanov","sequence":"first","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2000,3,9]]},"reference":[{"key":"e_1_2_1_2_1","volume-title":"The biology of heat shock proteins and molecular chaperones","author":"Morimoto R.I.","year":"1994"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(92)90439-G"},{"key":"e_1_2_1_4_1","first-page":"191","volume-title":"Stress proteins in biology and medicine","author":"Georgopoulos C.","year":"1990"},{"key":"e_1_2_1_5_1","first-page":"7903","volume-title":"Proc. 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