{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T12:05:16Z","timestamp":1770897916951,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1999,11,30]],"date-time":"1999-11-30T00:00:00Z","timestamp":943920000000},"content-version":"vor","delay-in-days":1380,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1996,2,19]]},"abstract":"<jats:p>Cysteine string proteins are putative synaptic vesicle proteins that lack a transmembrane domain. Our analysis shows that <jats:italic>Drosophila<\/jats:italic> cysteine string proteins are extensively modified by hydroxylamine\u2010sensitive fatty acylation. This modification could be responsible for association of csp's with membranes. Extensive deacylation of Dcsp's by a 20 h incubation in 1 M hydroxylamine, pH 7.0, or methanolic KOH produces a protein of 6\u20137 kDa lower mass than untreated Dcsp's. Surprisingly, the hydroxylamine treatment does not cause release of Dcsp's from membranes. On the other hand, alkaline stripping of membranes isolated from <jats:italic>Drosophila<\/jats:italic> brain by 0.1 M sodium carbonate, pH 11.5, causes a significant release of Dcsp's from membranes into the cytosol. These results indicate that fatty acylation may not form the main anchor of Dcsp's in membranes. Taking advantage of the endocytotic block in the <jats:italic>Drosophila<\/jats:italic> mutant <jats:italic>shibire<\/jats:italic>\n<jats:sup>ts1<\/jats:sup>, we analyzed the acylation states of Dcsp's in two stages during synaptic vesicle recycling and found no evidence for an acylation\/ deacylation cycle of Dcsp's in the brain nerve terminals.<\/jats:p>","DOI":"10.1016\/0014-5793(96)00026-9","type":"journal-article","created":{"date-parts":[[2003,4,30]],"date-time":"2003-04-30T22:24:07Z","timestamp":1051741447000},"page":"251-256","source":"Crossref","is-referenced-by-count":32,"title":["Association of <i>Drosophila<\/i> cysteine string proteins with membranes"],"prefix":"10.1002","volume":"380","author":[{"given":"Jana","family":"van de Goor","sequence":"first","affiliation":[]},{"given":"Regis B.","family":"Kelly","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1999,11,30]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90190-O"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7906056"},{"key":"e_1_2_1_4_1","first-page":"5739","volume-title":"Proc. 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