{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:17:24Z","timestamp":1779344244496,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[1999,11,30]],"date-time":"1999-11-30T00:00:00Z","timestamp":943920000000},"content-version":"vor","delay-in-days":1359,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1996,3,11]]},"abstract":"<jats:p>Mitochondrial free Ca<jats:sup>2+<\/jats:sup> may regulate mitochondrial ATP production during cardiac exercise. Here, using laser scanning confocal microscopy of adult rabbit cardiac myocytes co\u2010loaded with Fluo\u20103 to measure free Ca<jats:sup>2+<\/jats:sup> and tetramethylrhodamine methylester to identify mitochondria, we measured cytosolic and mitochondrial Ca<jats:sup>2+<\/jats:sup> transients during the contractile cycle. In resting cells, cytosolic and mitochondrial Fluo\u20103 signals were similar. During electrical pacing, transients of Fluo\u20103 fluorescence occurred in both the cytosolic and mitochondrial compartments. Both the mitochondrial and the cytosolic transients were potentiated by isoproterenol. These experiments show directly that mitochondrial free Ca<jats:sup>2+<\/jats:sup> rises and falls during excitation\u2010contraction coupling in cardiac myocytes and that changes of mitochondrial Ca<jats:sup>2+<\/jats:sup> are kinetically component to regulate mitochondrial metabolism on a beat\u2010to\u2010beat basis.<\/jats:p>","DOI":"10.1016\/0014-5793(96)00138-x","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T17:55:22Z","timestamp":1027619722000},"page":"31-36","source":"Crossref","is-referenced-by-count":94,"title":["Mitochondrial free calcium transients during excitation\u2010contraction coupling in rabbit cardiac myocytes"],"prefix":"10.1002","volume":"382","author":[{"given":"Enrique","family":"Chacon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John J","family":"Lemasters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisayuki","family":"Ohata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian S","family":"Harper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donna R","family":"Trollinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Herman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1999,11,30]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.267.2.C313"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1994.74.3.595"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000111332"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00762734"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470122624.ch2"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(73)91437-X"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)34194-7"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2828(80)90058-9"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)43871-4"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(83)80421-9"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3704638"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1989.256.1.H265"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(80)80986-0"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2140581"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2410145"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1991.261.6.C1123"},{"key":"e_1_2_1_18_1","first-page":"H1203","volume":"265","author":"Leisey J.R","year":"1993","journal-title":"Am. 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