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Under denaturing conditions the protein split into 2 components which migrated at 45 and 50 kDa in SDS\u2010PAGE, suggesting that the native enzyme is a heterodimer. The purified enzyme was characterized in terms of physicochemical and kinetic properties, and substrate specificity. It was specific for histone H4, leading to acetylation of non\u2010acetylated H4 subspecies into the di\u2010acetylated state in vitro. Its activity was coincident with the intensity of DNA replication in meristematic cells during embryo germination. 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