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The 5\u2032 untranslated region from SV40 early genes (SU) which was unable to stimulate translation of second cistrons amplified markedly the internal ribosome entry site (IRES) effect of the HTLV\u20101 \u2018R\u2019 fragments. The \u2018R\u2019 regions from HTLV\u20101 have therefore properties similar to internal ribosome entry sites (IRES) originally found in picornavirus. The beginning of the U5 region from HTLV\u20101 contains a polypyrimidine sequence which is known to play an essential role in the IRES activity in picornavirus. The same experiments carried out using the \u2018R\u2019 region from bovine leukaemia virus (BLV) showed that this sequence has at most a weak IRES effect. One retroviruses HTLV\u20101 and perhaps others contain therefore an IRES activity. Interestingly, the combined SU \u2018R\u2019 sequence worked efficiently with different cistrons, different promoters and in all tested cell lines, whereas the poliovirus IRES was active in CHO cells but not in the mouse mammary cell line HC11. The SU \u2018R\u2019 sequence may therefore preferably be used to generate active bicistronic mRNAs.<\/jats:p>","DOI":"10.1016\/0014-5793(96)00815-0","type":"journal-article","created":{"date-parts":[[2003,4,23]],"date-time":"2003-04-23T20:36:00Z","timestamp":1051130160000},"page":"220-224","source":"Crossref","is-referenced-by-count":33,"title":["The RU5 (\u2018R\u2019) region from human leukaemia viruses (HTLV\u20101) contains an internal ribosome entry site (IRES)\u2010like sequence"],"prefix":"10.1002","volume":"392","author":[{"given":"Jo\u00e9","family":"Attal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie-Claire","family":"Th\u00e9ron","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fr\u00e9d\u00e9rique","family":"Taboit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Cajero-Juarez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy","family":"Kann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Bolifraud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louis Marie","family":"Houdebine","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1999,3,23]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.00747.x"},{"key":"e_1_2_1_3_1","unstructured":"1995 M. 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