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Replacement of the \u03b3\u2010 or \u03b5\u2010site significantly suppressed secretion of amyloid \u03b2\u2010protein (A\u03b2), and produced longer A\u03b2 or longer APP intracellular domain, respectively. This cleavage at the midportion between \u03b3\u2010 and \u03b5\u2010sites was also \u03b3\u2010secretase\u2010dependent. Blocking this cleavage with a Trp stretch remarkably suppressed A\u03b2 generation, indicating that the midportion cleavage is required for the generation of A\u03b2.<\/jats:p>","DOI":"10.1016\/j.febslet.2005.04.037","type":"journal-article","created":{"date-parts":[[2005,4,30]],"date-time":"2005-04-30T11:22:07Z","timestamp":1114860127000},"page":"2907-2912","source":"Crossref","is-referenced-by-count":24,"title":["Blocking the cleavage at midportion between \u03b3\u2010 and \u03b5\u2010sites remarkably suppresses the generation of amyloid \u03b2\u2010protein"],"prefix":"10.1002","volume":"579","author":[{"given":"Toru","family":"Sato","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Tanimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoko","family":"Hirotani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takaomi C.","family":"Saido","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maho","family":"Morishima-Kawashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuo","family":"Ihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2001.81.2.741"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kve180"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C100357200"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C100410200"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi015794o"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211161200"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0861-04.2004"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.05.051"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.36.21914"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi971071m"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.6.3053"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.17.11914"},{"key":"e_1_2_6_14_1","first-page":"45","article-title":"Prediction of the secondary structure of proteins from their amino acid sequence","volume":"47","author":"Chou P.Y.","year":"1978","journal-title":"Adv. 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