{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T04:36:18Z","timestamp":1774067778763,"version":"3.50.1"},"reference-count":54,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2005,7,26]],"date-time":"2005-07-26T00:00:00Z","timestamp":1122336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2005,8,29]]},"abstract":"<jats:p>We propose that the proton pump is operated by redox\u2010driven conformational changes of the quinone binding protein. In the input state, semiquinone is reduced to quinol, acquiring two protons from the N (matrix) side of the mitochondrial inner membrane and an electron from the low potential (NADH) side of the respiratory chain. A conformational change brings the protons into position for release at the P (inter\u2010membrane space) side of the membrane via a proton\u2010well. Concomitantly, an electron is donated to the quinone pool at the high potential side of the coupling site. The system then returns to the original state to repeat the cycle. This hypothesis provides a useful frame work for further investigation of the mechanism of proton translocation in complex I.<\/jats:p>","DOI":"10.1016\/j.febslet.2005.06.086","type":"journal-article","created":{"date-parts":[[2005,7,28]],"date-time":"2005-07-28T21:24:29Z","timestamp":1122585869000},"page":"4555-4561","source":"Crossref","is-referenced-by-count":85,"title":["Conformation\u2010driven and semiquinone\u2010gated proton\u2010pump mechanism in the NADH\u2010ubiquinone oxidoreductase (complex I)"],"prefix":"10.1002","volume":"579","author":[{"given":"Tomoko","family":"Ohnishi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John C.","family":"Salerno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,7,26]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1991.tb15945.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S003358350000425X"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(98)00027-9"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(84)80338-5"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00575-X"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1997.1518"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.40.28598"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0026977"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/jtbi.1996.0387"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00222a021"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02417-6"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/373580a0"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108796200"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.11.107"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1016083419979"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi048132i"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)00391-0"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi049444o"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(00)00065-7"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi048348k"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0269660"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302361200"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0340346"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(97)00009-1"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.7.4149"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.3509"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1976.tb10277.x"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-5193(76)90054-0"},{"key":"e_1_2_8_30_1","first-page":"285","volume-title":"Iron\u2013Sulfur Proteins","author":"Ohnishi T.","year":"1982"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)00803-H"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi9612982"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(98)00031-0"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(96)00141-7"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.5520090203"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00181a018"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi000335t"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi027158b"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)00387-9"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2728(79)90010-0"},{"key":"e_1_2_8_41_1","unstructured":"P. Mitchell 1968 Glynn Research Bodmin"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.388618"},{"key":"e_1_2_8_43_1","unstructured":"D.G. Nicholls S.J. Ferguson 2002 Academic Press Inc. London"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00049a012"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi000842a"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M208849200"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M308967200"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.20155"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi025525d"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi049927s"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(74)90672-X"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi050134v"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.5.2625"},{"key":"e_1_2_8_54_1","unstructured":"M. Wikstr\u00f6m K. Krab M. Saraste 1981 Academic Press London"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4173(84)90007-7"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.febslet.2005.06.086","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.febslet.2005.06.086","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T03:06:59Z","timestamp":1697080019000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/j.febslet.2005.06.086"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,7,26]]},"references-count":54,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2005,8,29]]}},"alternative-id":["10.1016\/j.febslet.2005.06.086"],"URL":"https:\/\/doi.org\/10.1016\/j.febslet.2005.06.086","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,7,26]]}}}