{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T19:08:12Z","timestamp":1774379292326,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2007,5,25]],"date-time":"2007-05-25T00:00:00Z","timestamp":1180051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2007,6,26]]},"abstract":"<jats:p>Plant legumains are cysteine proteinases putatively involved in processing endogenous proteins. Phytocystatins (PhyCys) have been described as plant inhibitors of papain\u2010like cysteine proteinases. Some PhyCys contain a carboxy terminal extension with an amino acid motif (SNSL) similar to that involved in the inhibition of legumain\u2010like proteins by human cystatins. The role of these carboxy terminal extended PhyCys as inhibitors of legumain\u2010like cysteine proteinases is here shown by in vitro inhibition of human legumain and legumain\u2010like activities from barley extracts. Moreover, site\u2010directed mutagenesis has demonstrated that the asparagine of the SNSL motif is essential in this inhibition. We prove for first time the existence of legumain inhibitors in plants.<\/jats:p>","DOI":"10.1016\/j.febslet.2007.05.042","type":"journal-article","created":{"date-parts":[[2007,5,26]],"date-time":"2007-05-26T11:11:02Z","timestamp":1180177862000},"page":"2914-2918","source":"Crossref","is-referenced-by-count":96,"title":["Carboxy terminal extended phytocystatins are bifunctional inhibitors of papain and legumain cysteine proteinases"],"prefix":"10.1002","volume":"581","author":[{"given":"Manuel","family":"Martinez","sequence":"first","affiliation":[]},{"given":"Mercedes","family":"Diaz-Mendoza","sequence":"additional","affiliation":[]},{"given":"Laura","family":"Carrillo","sequence":"additional","affiliation":[]},{"given":"Isabel","family":"Diaz","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2007,5,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.12.8090"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1360-1385(02)02298-7"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(91)81349-D"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1998.0875"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.0609h.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eri172"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0795-658"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0240274"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(00)89080-5"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erl200"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.2003.16.10.876"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1010697204686"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/86188"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1993.1274"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)53726-1"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)01574-9"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.27.19195"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M411914200"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006170518002"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-003-1024-5"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erh247"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1099859"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2004.09.056"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2Fj.febslet.2007.05.042","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/j.febslet.2007.05.042","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T20:13:56Z","timestamp":1697141636000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/j.febslet.2007.05.042"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5,25]]},"references-count":24,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2007,6,26]]}},"alternative-id":["10.1016\/j.febslet.2007.05.042"],"URL":"https:\/\/doi.org\/10.1016\/j.febslet.2007.05.042","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,5,25]]}}}