{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T13:35:05Z","timestamp":1740144905076,"version":"3.37.3"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T00:00:00Z","timestamp":1715299200000},"content-version":"vor","delay-in-days":4422,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100002367","name":"Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["KSCX2-EW-R-01-04"],"award-info":[{"award-number":["KSCX2-EW-R-01-04"]}],"id":[{"id":"10.13039\/501100002367","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Science and Technology of China","award":["2011CB944100"],"award-info":[{"award-number":["2011CB944100"]}]},{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"publisher","award":["90919024","30900831"],"award-info":[{"award-number":["90919024","30900831"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Histone H3 lysine 4 trimethylation (H3K4me3) is well known to occur in the promoter region of genes for transcription activation. However, when investigating the H3K4me3 profiles in the mouse cerebrum and testis, we discovered that H3K4me3 also has a significant enrichment at the 3\u2032 end of actively transcribed (sense) genes, named as 3\u2032-H3K4me3. 3\u2032-H3K4me3 is associated with \u223c15% of protein-coding genes in both tissues. In addition, we examined the transcriptional initiation signals including RNA polymerase II (RNAPII) binding sites and 5\u2032-CAGE-tag that marks transcriptional start sites. Interestingly, we found that 3\u2032-H3K4me3 is associated with the initiation of antisense transcription. Furthermore, 3\u2032-H3K4me3 modification levels correlate positively with the antisense expression levels of the associated sense genes, implying that 3\u2032-H3K4me3 is involved in the activation of antisense transcription. Taken together, our findings suggest that H3K4me3 may be involved in the regulation of antisense transcription that initiates from the 3\u2032 end of sense genes. In addition, a positive correlation was also observed between the expression of antisense and the associated sense genes with 3\u2032-H3K4me3 modification. More importantly, we observed the 3\u2032-H3K4me3 enrichment among genes in human, fruitfly and Arabidopsis, and found that the sequences of 3\u2032-H3K4me3-marked regions are highly conserved and essentially indistinguishable from known promoters in vertebrate. Therefore, we speculate that these 3\u2032-H3K4me3-marked regions may serve as potential promoters for antisense transcription and 3\u2032-H3K4me3 appear to be a universal epigenetic feature in eukaryotes. Our results provide a novel insight into the epigenetic roles of H3K4me3 and the regulatory mechanism of antisense transcription.<\/jats:p>","DOI":"10.1016\/j.gpb.2012.05.001","type":"journal-article","created":{"date-parts":[[2012,6,9]],"date-time":"2012-06-09T05:50:14Z","timestamp":1339221014000},"page":"74-81","source":"Crossref","is-referenced-by-count":11,"title":["The Association Between H3K4me3 and Antisense Transcription"],"prefix":"10.1093","volume":"10","author":[{"given":"Peng","family":"Cui","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing 100029 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanfei","family":"Liu","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences , Beijing 100029 , China"},{"name":"Graduate University of 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