{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T15:57:34Z","timestamp":1718639854566},"reference-count":50,"publisher":"Wiley","issue":"2-3","license":[{"start":{"date-parts":[[2000,2,7]],"date-time":"2000-02-07T00:00:00Z","timestamp":949881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2000,2,11]]},"abstract":"<jats:p>The data presented implicate a GATA binding site in the transcriptional regulation of 15\u2010lipoxygenase\u20101 (15\u2010LO\u20101) gene expression in human colorectal carcinoma Caco\u20102 cells. High expression of GATA\u20106 mRNA and protein was observed, while GATA\u20104 mRNA was expressed at a very low level in Caco\u20102 cells. The expression of GATA\u20106 was down\u2010regulated, while 15\u2010LO\u20101 expression was dramatically up\u2010regulated after treatment with sodium butyrate (NaBT). A study using an electrophoretic mobility shift assay indicated that a GATA binding site of the 15\u2010LO\u20101 promoter region binds to GATA proteins present in both undifferentiated and, to a lesser extent, NaBT\u2010treated (differentiated) Caco\u20102 cells. Moreover, that DNA binding shift band was disrupted after the addition of GATA\u20106 antibody in a supershift assay in the absence of NaBT, suggesting that GATA\u20106 is bound to the GATA binding site of the 15\u2010LO\u20101 promoter in undifferentiated cells. In contrast, the addition of GATA\u20106 antibody did not affect the DNA binding ability in NaBT\u2010induced differentiated cells. On the other hand, mutation of the GATA site of the 15\u2010LO\u20101 promoter decreased the transactivation of the 15\u2010LO\u20101 promoter as measured by luciferase activity in both FBS and NaBT cultured cells, indicating an unknown GATA binding protein to up\u2010regulate 15\u2010LO\u20101 expression. These implicate the GATA site at \u2212240 of the proximal region of the 15\u2010LO\u20101 promoter in the basic transcription of 15\u2010LO\u20101 gene expression in Caco\u20102 cells, with GATA\u20106 acting to repress 15\u2010LO\u20101 expression.<\/jats:p>","DOI":"10.1016\/s0014-5793(00)01155-8","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T12:18:27Z","timestamp":1027599507000},"page":"341-347","source":"Crossref","is-referenced-by-count":24,"title":["A GATA binding site is involved in the regulation of 15\u2010lipoxygenase\u20101 expression in human colorectal carcinoma cell line, Caco\u20102"],"prefix":"10.1002","volume":"467","author":[{"given":"Hideki","family":"Kamitani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideto","family":"Kameda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uddhav P.","family":"Kelavkar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas E.","family":"Eling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2000,2,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/338435a0"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/371221a0"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)31636-8"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4781(97)00049-3"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.13.4.2235"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1996.0165"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1996.8485"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.22.10876"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)80184-3"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)70037-4"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.5.2901"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.118.3.817"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0012-1606(98)80006-7"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.24.4909"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1998.274.2.G314"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.4.723"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7827(97)00002-7"},{"key":"e_1_2_6_19_1","first-page":"L392","volume":"262","author":"Sigal E.","year":"1992","journal-title":"Am. 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