{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T08:37:48Z","timestamp":1762504668042},"reference-count":20,"publisher":"Wiley","issue":"2-3","license":[{"start":{"date-parts":[[2000,4,27]],"date-time":"2000-04-27T00:00:00Z","timestamp":956793600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2000,4,28]]},"abstract":"<jats:p>Mammalian tRNA 3\u2032 processing endoribonuclease (3\u2032 tRNase) is an enzyme responsible for the removal of a 3\u2032 trailer from pre\u2010tRNA. The enzyme can also recognize and cleave any target RNA that forms a pre\u2010tRNA\u2010like complex with another RNA. To investigate the interaction between 3\u2032 tRNase and substrates, we tested various anomalous pre\u2010tRNA\u2010like complexes for cleavage by pig 3\u2032 tRNase. We examined how base mismatches in the acceptor stem affect 3\u2032 tRNase cleavage of RNA complexes, and found that even one base mismatch in the acceptor stem drastically reduces the cleavage efficiency. Mammalian 3\u2032 tRNase was able to recognize complexes between target RNAs and 5\u2032\u2010half tDNAs, and cleave the target RNAs, although inefficiently, whereas the enzyme had no activity to cleave phosphodiester bonds of DNA. A relatively long RNA target, the <jats:italic>Escherichia coli<\/jats:italic> chloramphenicol acetyltransferase (CAT) mRNA, was cleaved by 3\u2032 tRNase in the presence of appropriate 5\u2032\u2010half tRNAs. We also demonstrated that an RNA complex of <jats:italic>lin\u20104<\/jats:italic> and <jats:italic>lin\u201014<\/jats:italic> from <jats:italic>Caenorhabditis elegans<\/jats:italic> can be recognized and cleaved by pig 3\u2032 tRNase.<\/jats:p>","DOI":"10.1016\/s0014-5793(00)01462-9","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T13:55:25Z","timestamp":1027605325000},"page":"179-186","source":"Crossref","is-referenced-by-count":30,"title":["Anomalous RNA substrates for mammalian tRNA 3\u2032 processing endoribonuclease"],"prefix":"10.1002","volume":"472","author":[{"given":"Masayuki","family":"Nashimoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2000,4,27]]},"reference":[{"key":"e_1_2_5_2_1","unstructured":"Sprague K.U. (1995) in: tRNA: Structure Biosynthesis and Function (S&#x00F6;ll D. and RajBhandary U.L. 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