{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T23:37:39Z","timestamp":1780097859202,"version":"3.54.0"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2000,5,12]],"date-time":"2000-05-12T00:00:00Z","timestamp":958089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2000,5,19]]},"abstract":"<jats:p>A recombinant soluble form of the catalytic domain of human ADAM\u201010 was expressed as an Fc fusion protein from myeloma cells. The ADAM\u201010 was catalytically active, cleaving myelin basic protein and peptides based on the previously described \u2018metallosheddase\u2019 cleavage sites of tumour necrosis factor \u03b1, CD40 ligand and amyloid precursor protein. The myelin basic protein degradation assay was used to demonstrate that hydroxamate inhibitors of matrix metalloproteinases (MMPs) were also inhibitors of ADAM\u201010. The natural MMP inhibitors, TIMP\u20102 and TIMP\u20104 were unable to inhibit ADAM\u201010, but TIMP\u20101 and TIMP\u20103 were inhibitory. Using a quenched fluorescent substrate assay and ADAM\u201010 we obtained approximate apparent inhibition constants of 0.1 nM (TIMP\u20101) and 0.9 nM (TIMP\u20103). The TIMP\u20101 inhibition of ADAM\u201010 could therefore prove useful in distinguishing its activity from that of TACE, which is only inhibited by TIMP\u20103, in cell based assays.<\/jats:p>","DOI":"10.1016\/s0014-5793(00)01528-3","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T02:34:39Z","timestamp":1027650879000},"page":"275-279","source":"Crossref","is-referenced-by-count":335,"title":["The in vitro activity of ADAM\u201010 is inhibited by TIMP\u20101 and TIMP\u20103"],"prefix":"10.1002","volume":"473","author":[{"given":"Augustin","family":"Amour","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.Graham","family":"Knight","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ailsa","family":"Webster","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick M.","family":"Slocombe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul E.","family":"Stephens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Kn\u00e4uper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.P.","family":"Docherty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gillian","family":"Murphy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2000,5,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80519-X"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(96)80111-6"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(98)80042-2"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020692710029"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560040502"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/385729a0"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/385733a0"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3210265"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.7.3408"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)01031-X"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.5.2810"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1998.00754.x"},{"key":"e_1_2_5_14_1","first-page":"4001","volume":"58","author":"Lombard M.A.","year":"1998","journal-title":"Cancer Res."},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19980501)69:2<143::AID-JCB5>3.0.CO;2-S"},{"key":"e_1_2_5_16_1","first-page":"1196","volume":"59","author":"Codony-Servat J.","year":"1999","journal-title":"Cancer Res."},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3170045"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.39.24588"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.7.3922"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(96)01410-X"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(95)48025-0"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8485"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5398.91"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00067a023"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)80300-6"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)88226-X"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830250639"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5420.1664"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2FS0014-5793%2800%2901528-3","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2FS0014-5793(00)01528-3","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/S0014-5793%2800%2901528-3","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T09:19:57Z","timestamp":1694855997000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/S0014-5793%2800%2901528-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,5,12]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2000,5,19]]}},"alternative-id":["10.1016\/S0014-5793(00)01528-3"],"URL":"https:\/\/doi.org\/10.1016\/s0014-5793(00)01528-3","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,5,12]]}}}