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The expressed and purified Tat\u2013SOD fusion protein in <jats:italic>Escherichia coli<\/jats:italic> can enter HeLa cells in a time\u2010 and dose\u2010dependent manner when added exogenously in a culture media. Denatured Tat\u2013SOD protein was transduced much more efficiently into cells than were native proteins. Once inside the cells, transduced Tat\u2013SOD protein was enzymatically active and stable for 24 h. The cell viability of HeLa cells treated with paraquat, an intracellular superoxide anion generator, was increased by transduced Tat\u2013SOD. These lines of results suggest that the transduction of Tat\u2013SOD fusion protein may be one of the ways to replenish the Cu,Zn\u2010SOD in the various disorders related to this antioxidant enzyme.<\/jats:p>","DOI":"10.1016\/s0014-5793(00)02215-8","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T20:46:08Z","timestamp":1027629968000},"page":"163-167","source":"Crossref","is-referenced-by-count":113,"title":["Transduction of Cu,Zn\u2010superoxide dismutase mediated by an HIV\u20101 Tat protein basic domain into mammalian cells"],"prefix":"10.1002","volume":"485","author":[{"given":"Hyeok Yil","family":"Kwon","sequence":"first","affiliation":[]},{"given":"Won Sik","family":"Eum","sequence":"additional","affiliation":[]},{"given":"Hyun Woo","family":"Jang","sequence":"additional","affiliation":[]},{"given":"Jung Hoon","family":"Kang","sequence":"additional","affiliation":[]},{"given":"Jiyoon","family":"Ryu","sequence":"additional","affiliation":[]},{"given":"Byung","family":"Ryong Lee","sequence":"additional","affiliation":[]},{"given":"Li Hua","family":"Jin","sequence":"additional","affiliation":[]},{"given":"Jinseu","family":"Park","sequence":"additional","affiliation":[]},{"given":"Soo Young","family":"Choi","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2000,11,21]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.4.9.2189775"},{"key":"e_1_2_5_3_1","unstructured":"Halliwell B. and Gutteridge J.M.C. 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