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Evidence suggests that both modifications are involved in regulating imprinted genes \u2013 a subset of genes whose expression depends on their parental origin. Using immunoprecipitation assays, we investigate the relationship between DNA methylation, histone H4 acetylation and gene expression in the well\u2010characterised imprinted <jats:italic>Igf2\u2010H19<\/jats:italic> domain on mouse chromosome 7. A systematic regional analysis of the acetylation status of the domain shows that parental\u2010specific differences in acetylation of the core histone H4 are present in the promoter regions of both <jats:italic>Igf2<\/jats:italic> and <jats:italic>H19<\/jats:italic> genes, with the expressed alleles being more acetylated than the silent alleles. A correlation between DNA methylation, histone hypoacetylation and gene repression is evident only at the promoter region of the <jats:italic>H19<\/jats:italic> gene. Treatment with trichostatin A, a specific inhibitor of histone deacetylase, reduces the expression of the active maternal <jats:italic>H19<\/jats:italic> allele and this can be correlated with regional changes in acetylation within the upstream regulatory domain. The data suggest that histone H4 acetylation and DNA methylation have distinct functions on the maternal and paternal <jats:italic>Igf2\u2010H19<\/jats:italic> domains.<\/jats:p>","DOI":"10.1016\/s0014-5793(00)02349-8","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T17:17:04Z","timestamp":1027617424000},"page":"165-169","source":"Crossref","is-referenced-by-count":63,"title":["Relationship between DNA methylation, histone H4 acetylation and gene expression in the mouse imprinted <i>Igf2\u2010H19<\/i> domain"],"prefix":"10.1002","volume":"488","author":[{"given":"Valerie","family":"Grandjean","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"O'Neill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Sado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryan","family":"Turner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Ferguson-Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,1,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/12664"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.4.1394-1406.2000"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/71750"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.31.1.493"},{"key":"e_1_2_6_6_1","first-page":"145","volume":"44","author":"Reik W.","year":"2000","journal-title":"Int. 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