{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T06:40:41Z","timestamp":1772088041231,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,2,22]],"date-time":"2001-02-22T00:00:00Z","timestamp":982800000000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2001,1,26]]},"abstract":"<jats:p>The SH3 domain of Bruton's tyrosine kinase (Btk) is preceded by the Tec homology (TH) region containing proline\u2010rich sequences. We have studied a protein fragment containing both the Btk SH3 domain and the proline\u2010rich sequences of the TH region (PRR\u2010SH3). Intermolecular NMR cross\u2010relaxation measurements, gel permeation chromatography profiles, titrations with proline\u2010rich peptides, and <jats:sup>15<\/jats:sup>N NMR relaxation measurements are all consistent with a monomer\u2013dimer equilibrium with a dissociation constant on the order of 60 \u03bcM. The intermolecular interactions do, at least in part, involve proline\u2010rich sequences in the TH region. This behavior of Btk PRR\u2010SH3 may have implications for the functional action of Btk.<\/jats:p>","DOI":"10.1016\/s0014-5793(00)02438-8","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T19:52:46Z","timestamp":1027626766000},"page":"67-70","source":"Crossref","is-referenced-by-count":33,"title":["Intermolecular interactions between the SH3 domain and the proline\u2010rich TH region of Bruton's tyrosine kinase"],"prefix":"10.1002","volume":"489","author":[{"given":"Henrik","family":"Hansson","sequence":"first","affiliation":[]},{"given":"Michael P","family":"Okoh","sequence":"additional","affiliation":[]},{"given":"C.I.Edvard","family":"Smith","sequence":"additional","affiliation":[]},{"given":"Mauno","family":"Vihinen","sequence":"additional","affiliation":[]},{"given":"Torleif","family":"H\u00e4rd","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,2,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/361226a0"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90667-F"},{"key":"e_1_2_6_4_1","unstructured":"Smith C.I.E. 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