{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T15:06:50Z","timestamp":1700320010507},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,8,14]],"date-time":"2001-08-14T00:00:00Z","timestamp":997747200000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2001,8,10]]},"abstract":"<jats:p>The hepatitis C virus (HCV) protease genes (NS2\/3 and NS3) were expressed in yeast with their natural substrates fused to a ligand\u2010dependent transcriptional activator, the retinoic acid receptor (RAR\u03b2). RAR\u03b2 can activate transcription in yeast cells in response to retinoic acids. We hypothesized that <jats:italic>cis<\/jats:italic>\u2010cleavage at the NS2\u20133 or NS3\u20134A junctions by the appropriate HCV proteases would release RAR\u03b2, thereby activating transcription of a reporter gene. Our results from Western blot analyses and reporter gene activation indicate that the wild\u2010type NS2\/3 and NS3 enzymes are catalytically active in yeast cells, whereas mutations in the catalytic domain of NS2(C993V) and NS3(S1165A) lead to inactive enzymes. We conclude that HCV NS2\/3 and NS3 protease activities can be reconstituted in yeast.<\/jats:p>","DOI":"10.1016\/s0014-5793(01)02678-3","type":"journal-article","created":{"date-parts":[[2002,10,14]],"date-time":"2002-10-14T15:56:21Z","timestamp":1034610981000},"page":"13-18","source":"Crossref","is-referenced-by-count":8,"title":["Reconstitution of hepatitis C virus protease activities in yeast"],"prefix":"10.1002","volume":"503","author":[{"given":"Paul","family":"Mak","sequence":"first","affiliation":[]},{"given":"Olga","family":"Palant","sequence":"additional","affiliation":[]},{"given":"Patrick","family":"Labont\u00e9","sequence":"additional","affiliation":[]},{"given":"Steve","family":"Plotch","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,8,14]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2523562"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.65.3.1105-1113.1991"},{"key":"e_1_2_4_4_1","first-page":"2831","volume":"67","author":"Grakoui A","year":"1993","journal-title":"J. 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