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Here we show NTF:NTF, CTF:CTF and NTF:CTF interactions by yeast two\u2010hybrid and in vivo endoplasmic reticulum split\u2010ubiquitin assays. Our results also highlight the involvement of HL1 \u2013 the hydrophilic loop between TMI and TMII \u2013 in the NTF:NTF binding site. Besides, nine FAD\u2010linked presenilin mutations substantially affected HL1:HL1 binding. From the evidence of NTF and CTF homodimerization, we propose the contribution of two NTFs and two CTFs, instead of a single NTF:CTF heterodimer, to the functional presenilin\u2013\u03b3\u2010secretase complex and that FAD mutations affect the assembly or stability of this complex.<\/jats:p>","DOI":"10.1016\/s0014-5793(01)02785-5","type":"journal-article","created":{"date-parts":[[2002,10,14]],"date-time":"2002-10-14T15:56:01Z","timestamp":1034610961000},"page":"81-86","source":"Crossref","is-referenced-by-count":43,"title":["Homodimerization of presenilin N\u2010terminal fragments is affected by mutations linked to Alzheimer's disease"],"prefix":"10.1002","volume":"505","author":[{"given":"Sara","family":"Cervantes","sequence":"first","affiliation":[]},{"given":"Roser","family":"Gonz\u00e0lez-Duarte","sequence":"additional","affiliation":[]},{"given":"Gemma","family":"Marfany","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,8,20]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s000180050219"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1004(1998)11:3<183::AID-HUMU1>3.0.CO;2-J"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/5.Supplement_1.1449"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s000180050035"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(00)00028-4"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.39.24536"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80291-3"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.45.28415"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.26.16470"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.6.3205"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/nbdi.1998.0171"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.10.5090"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.20.13818"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)61517-6"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.3109\/01677069809108554"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(93)80043-T"},{"key":"e_1_2_5_18_1","unstructured":"Ausubel F.M. 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