{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T15:35:05Z","timestamp":1775662505378,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,11,27]],"date-time":"2001-11-27T00:00:00Z","timestamp":1006819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2001,12,7]]},"abstract":"<jats:p>The first step in intestinal iron absorption is mediated by the H<jats:sup>+<\/jats:sup>\u2010coupled Fe<jats:sup>2+<\/jats:sup> transporter called divalent cation transporter 1\/divalent metal ion transporter 1 (DCT1\/DMT1) (also known as natural resistance\u2010associated macrophage protein 2). DCT1\/DMT1 mRNA levels in the duodenum strongly increase in response to iron depletion. To study the mechanism of iron\u2010dependent DCT1\/DMT1 mRNA regulation, we investigated the endogenous expression of DCT1\/DMT1 mRNA in various cell types. We found that only the iron responsive element (IRE)\u2010containing form, which corresponds to one of two splice forms of DCT1\/DMT1, is responsive to iron treatment and this responsiveness was cell type specific. We also examined the interaction of the putative 3\u2032\u2010UTR IRE with iron responsive binding proteins (IRP1 and IRP2), and found that IRP1 binds to the DCT1\/DMT1\u2010IRE with higher affinity compared to IRP2. This differential binding of IRP1 and IRP2 was also reported for the IREs of transferrin receptors, erythroid 5\u2010aminolevulinate synthase and mitochondrial aconitase. We propose that regulation of DCT1\/DMT1 mRNA by iron involves post\u2010transcriptional regulation through the binding of IRP1 to the transporter's IRE, as well as other as yet unknown factors.<\/jats:p>","DOI":"10.1016\/s0014-5793(01)03189-1","type":"journal-article","created":{"date-parts":[[2002,10,14]],"date-time":"2002-10-14T15:56:01Z","timestamp":1034610961000},"page":"309-316","source":"Crossref","is-referenced-by-count":260,"title":["Iron\u2010dependent regulation of the divalent metal ion transporter"],"prefix":"10.1002","volume":"509","author":[{"given":"Hiromi","family":"Gunshin","sequence":"first","affiliation":[]},{"given":"Charles R.","family":"Allerson","sequence":"additional","affiliation":[]},{"given":"Maria","family":"Polycarpou-Schwarz","sequence":"additional","affiliation":[]},{"given":"Andreas","family":"Rofts","sequence":"additional","affiliation":[]},{"given":"Jack T.","family":"Rogers","sequence":"additional","affiliation":[]},{"given":"Fumio","family":"Kishi","sequence":"additional","affiliation":[]},{"given":"Matthias W.","family":"Hentze","sequence":"additional","affiliation":[]},{"given":"Tracey A.","family":"Rouault","sequence":"additional","affiliation":[]},{"given":"Nancy C.","family":"Andrews","sequence":"additional","affiliation":[]},{"given":"Matthias A.","family":"Hediger","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,11,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2190001"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.31.19095"},{"key":"e_1_2_6_4_1","unstructured":"Brittenham G.M. 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