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First, Cdc48 prevents luciferase from aggregating following a heat shock. Second, it inhibits the aggregation of rhodanese upon dilution. Third, Cdc48 binds specifically to heat\u2010denatured luciferase. These chaperone\u2010like functions seem to be independent of ATPase activity. Furthermore, Cdc48 can act as a co\u2010chaperone in the Hsc70\u2013Hsp40 chaperone system. These results show that Cdc48 possesses chaperone\u2010like activities and can functionally interact with Hsc70. Cdc48's ability to recognise denatured proteins can also be a source of unspecific binding in biochemical interaction experiments.<\/jats:p>","DOI":"10.1016\/s0014-5793(02)02777-1","type":"journal-article","created":{"date-parts":[[2002,10,10]],"date-time":"2002-10-10T21:13:43Z","timestamp":1034284423000},"page":"107-110","source":"Crossref","is-referenced-by-count":51,"title":["Cdc48 can distinguish between native and non\u2010native proteins in the absence of cofactors"],"prefix":"10.1002","volume":"520","author":[{"given":"Sven","family":"Thoms","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,5,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.114.3.443"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90268-6"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.8.2529"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1201-1086"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90269-4"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90270-8"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00869.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.6.3562"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/35087056"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.12.10.3226"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00595-5"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/414652a"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.12.12.4114"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.2.626-634.2002"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/21.4.615"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb746"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.3.729"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80145-4"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(97)01212-9"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80327-X"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.7.2874"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.32.19617"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(95)00136-I"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)01232-0"},{"key":"e_1_2_6_26_1","first-page":"245","volume":"379","author":"Beissinger M.","year":"1998","journal-title":"Biol. 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