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More recently, the topological model of CydA was revised to predict nine transmembrane helices, based on additional sequence information from other organisms. In the current work, the topology of the <jats:italic>E. coli<\/jats:italic> oxidase was experimentally examined using \u03b2\u2010lactamase gene fusions. The results confirm the revised topology, which places the oxygen reactive site near the periplasmic surface.<\/jats:p>","DOI":"10.1016\/s0014-5793(04)00125-5","type":"journal-article","created":{"date-parts":[[2004,2,13]],"date-time":"2004-02-13T10:06:52Z","timestamp":1076666812000},"page":"58-62","source":"Crossref","is-referenced-by-count":36,"title":["Gene fusions with \u03b2\u2010lactamase show that subunit I of the cytochrome <i>bd<\/i> quinol oxidase from <i>E. coli<\/i> has nine transmembrane helices with the O<sub>2<\/sub> reactive site near the periplasmic surface"],"prefix":"10.1002","volume":"561","author":[{"given":"Jie","family":"Zhang","sequence":"first","affiliation":[]},{"given":"Blanca","family":"Barquera","sequence":"additional","affiliation":[]},{"given":"Robert B","family":"Gennis","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,2,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(97)00046-7"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.63.070194.003331"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(98)00171-6"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.18.5587-5600.1994"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr950051s"},{"key":"e_1_2_6_7_1","first-page":"79","volume":"2","author":"Mogi T.","year":"1998","journal-title":"J. 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