{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T00:02:00Z","timestamp":1771372920902,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"1-3","license":[{"start":{"date-parts":[[2004,2,13]],"date-time":"2004-02-13T00:00:00Z","timestamp":1076630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[2004,3,12]]},"abstract":"<jats:p>Isoprenoids are synthesised either through the classical, mevalonate pathway, or the alternative, non\u2010mevalonate, 2\u2010<jats:italic>C<\/jats:italic>\u2010methyl\u2010<jats:sc>D<\/jats:sc>\u2010erythritol 4\u2010phosphate (MEP) pathway. The latter is found in many microbial pathogens and proceeds via (<jats:italic>E<\/jats:italic>)\u20104\u2010hydroxy\u20103\u2010methyl\u2010but\u20102\u2010enyl pyrophosphate (HMB\u2010PP), a potent activator of human V\u03b39\/V\u03b42 T cells. <jats:italic>Listeria monocytogenes<\/jats:italic> is the only pathogenic bacterium known to contain both pathways concurrently. Strategic gene knockouts demonstrate that either pathway is functional but dispensable for viability. Yet, disrupting the mevalonate pathway results in a complementary upregulation of the MEP pathway. V\u03b39\/V\u03b42 T cell bioactivity is increased in \u0394<jats:italic>lytB<\/jats:italic> mutants where HMB\u2010PP accumulation is expected, and lost in \u0394<jats:italic>gcpE<\/jats:italic> mutants which fail to produce HMB\u2010PP.<\/jats:p>","DOI":"10.1016\/s0014-5793(04)00131-0","type":"journal-article","created":{"date-parts":[[2004,2,17]],"date-time":"2004-02-17T10:08:37Z","timestamp":1077012517000},"page":"99-104","source":"Crossref","is-referenced-by-count":61,"title":["The interplay between classical and alternative isoprenoid biosynthesis controls \u03b3\u03b4 T cell bioactivity of <i>Listeria monocytogenes<\/i>"],"prefix":"10.1002","volume":"561","author":[{"given":"M\u00e1ire","family":"Begley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cormac G.M.","family":"Gahan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann-Kristin","family":"Kollas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Hintz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colin","family":"Hill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hassan","family":"Jomaa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Eberl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,2,13]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002810000042"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)03191-X"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-894X(03)00138-0"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(02)03725-0"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(02)03726-2"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(03)00483-6"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4906(03)00170-4"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.10.4267-4272.1997"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0924-2244(91)90635-V"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0505.990502"},{"key":"e_1_2_5_12_1","unstructured":"Gahan C.G. and Hill C. (2003) in: Microbial Stress Adaptation and Food Safety (Yousef A.E. and Juneja V.K. Eds.) pp. 213&#x2013;245 CRC Press London."},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00775.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.14.3650-3656.1998"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-64.9.1362"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.6.2692-2698.2001"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.12.6005-6012.2002"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03080.x"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2002.01414.x"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.24.7011-7018.1995"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2003.tb11494.x"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-8749(02)00519-1"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0040-4039(96)01787-X"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.66.808"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3530059"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.6.3655"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1515\/znc-1998-11-1208"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.19.6.1013"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5433.1573"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200113584"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03403540"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.11.6421"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.9.5604"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2FS0014-5793%2804%2900131-0","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2FS0014-5793(04)00131-0","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/S0014-5793%2804%2900131-0","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T11:09:22Z","timestamp":1694862562000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/S0014-5793%2804%2900131-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,2,13]]},"references-count":32,"journal-issue":{"issue":"1-3","published-print":{"date-parts":[[2004,3,12]]}},"alternative-id":["10.1016\/S0014-5793(04)00131-0"],"URL":"https:\/\/doi.org\/10.1016\/s0014-5793(04)00131-0","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,2,13]]}}}