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Optimal peroxynitrite formation occurs at 70 \u03bcM oxygen with purine substrates. Kinetic parameters: <jats:italic>V<\/jats:italic>\n<jats:sub>max<\/jats:sub>\u223c50 nmol\/min\/mg and <jats:italic>K<\/jats:italic>\n<jats:sub>m<\/jats:sub> of 22, 36 and 70 \u03bcM for hypoxanthine, pterin and nitrite respectively. Peroxynitrite generation is inhibited by allopurinol, superoxide dismutase and diphenylene iodonium. A role for this enzyme activity can be found in the antibacterial activity of milk and circulating xanthine oxidase activity.<\/jats:p>","DOI":"10.1016\/s0014-5793(04)00218-2","type":"journal-article","created":{"date-parts":[[2004,3,6]],"date-time":"2004-03-06T10:39:45Z","timestamp":1078569585000},"page":"129-133","source":"Crossref","is-referenced-by-count":27,"title":["Peroxynitrite formation from the simultaneous reduction of nitrite and oxygen by xanthine oxidase"],"prefix":"10.1002","volume":"562","author":[{"given":"Timothy M.","family":"Millar","sequence":"first","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,3,5]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(00)00373-7"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1179\/135100002125000280"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/tx970160x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(99)00006-8"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.21.11853"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2002.tb04069.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(92)90434-X"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02660-X"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0895-804"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(89)91106-6"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1999.tb09253.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)56885-X"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00112.2001"},{"key":"e_1_2_5_15_1","first-page":"87","volume":"548","author":"Parks D.","year":"1986","journal-title":"Acta Physiol. 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