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When purified rat liver peroxisomes were incubated with 2\u2010hydroxy\u20103\u2010methylhexadecanoyl\u2010CoA 2\u2010methylpentadecanal was indeed formed. The production rates of formyl\u2010CoA (measured as formate) and of the aldehyde were in the same range. While the production of formate remained unaltered in the presence of NAD<jats:sup>+<\/jats:sup>, the amount of 2\u2010methylpentadecanal was decreased, which was accompanied by the formation of 2\u2010methylpentadecanoic acid. These data indicate that (1) during \u03b1\u2010oxidation the 2\u2010hydroxy\u20103\u2010methylacyl\u2010CoA is cleaved to a 2\u2010methyl\u2010branched aldehyde and formyl\u2010CoA and (2) liver peroxisomes are capable of converting this aldehyde to a 2\u2010methyl\u2010branched fatty acid.<\/jats:p>","DOI":"10.1016\/s0014-5793(97)00856-9","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T17:54:24Z","timestamp":1027619664000},"page":"643-645","source":"Crossref","is-referenced-by-count":37,"title":["Formation of a 2\u2010methyl\u2010branched fatty aldehyde during peroxisomal \u03b1\u2010oxidation"],"prefix":"10.1002","volume":"412","author":[{"given":"Kathleen","family":"Croes","sequence":"first","affiliation":[]},{"given":"Minne","family":"Casteels","sequence":"additional","affiliation":[]},{"given":"Stanny","family":"Asselberghs","sequence":"additional","affiliation":[]},{"given":"Piet","family":"Herdewijn","sequence":"additional","affiliation":[]},{"given":"Guy P","family":"Mannaerts","sequence":"additional","affiliation":[]},{"given":"Paul P","family":"Van Veldhoven","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1998,1,14]]},"reference":[{"key":"e_1_2_5_2_1","unstructured":"Steinberg D. in: Scriver C.R. 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