{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:21:58Z","timestamp":1779344518232,"version":"3.51.4"},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1998,8,28]],"date-time":"1998-08-28T00:00:00Z","timestamp":904262400000},"content-version":"vor","delay-in-days":375,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1997,8,18]]},"abstract":"<jats:p>The 14\u20103\u20103 protein family binds a variety of proteins in cell\u2010signaling pathways, but the structural elements necessary for the ligand binding are poorly understood. Here we demonstrate that the `box\u20101' region, which spans residues 171\u2013213 in the \u03b7\u2010isoform and was previously identified as the binding site of 14\u20103\u20103 to the phosphorylated tryptophan hydroxylase, plays a critical role in the interaction with many target proteins. Using a series of truncated 14\u20103\u20103 mutants, we show that the mutant 167\u2013213 carrying box\u20101 binds bacurovirus\u2010expressed Raf\u20101 and Bcr protein kinases to the similar extent as the full\u2010length 14\u20103\u20103 in a phosphorylation\u2010dependent manner, while the mutants lacking this region abolish the binding activity. Furthermore, the box\u20101 region also appears essential for binding of 14\u20103\u20103 to more than 40 phosphoproteins found in the brainstem extract. These results suggest that the box\u20101 region, consisting of helices 7 and 8 in the tertiary structure, is a common structural element whereby the 14\u20103\u20103 protein binds many, if not all, target proteins.<\/jats:p>","DOI":"10.1016\/s0014-5793(97)00910-1","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T15:03:33Z","timestamp":1027609413000},"page":"273-276","source":"Crossref","is-referenced-by-count":35,"title":["The 14\u20103\u20103 protein binds its target proteins with a common site located towards the C\u2010terminus"],"prefix":"10.1002","volume":"413","author":[{"given":"Tohru","family":"Ichimura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuki","family":"Ito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiharu","family":"Itagaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masuhiro","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuneyosi","family":"Horigome","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saburo","family":"Omata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeo","family":"Ohno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiaki","family":"Isobe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1998,8,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(92)90339-B"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0962-8924(96)10029-5"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)69215-X"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.19.7084"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.1796"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.15.6.3390"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81067-3"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.48.28515"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8085159"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/hon.2900120202"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7939633"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)41496-8"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.33.20029"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8085158"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81382-3"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(96)01188-X"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2FS0014-5793%2897%2900910-1","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2FS0014-5793(97)00910-1","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/S0014-5793%2897%2900910-1","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T02:43:09Z","timestamp":1694918589000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/S0014-5793%2897%2900910-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,8,18]]},"references-count":16,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,8,18]]}},"alternative-id":["10.1016\/S0014-5793(97)00910-1"],"URL":"https:\/\/doi.org\/10.1016\/s0014-5793(97)00910-1","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,8,18]]}}}