{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:44:52Z","timestamp":1761540292701},"reference-count":22,"publisher":"Wiley","issue":"1-2","license":[{"start":{"date-parts":[[1998,3,17]],"date-time":"1998-03-17T00:00:00Z","timestamp":890092800000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1998,3,6]]},"abstract":"<jats:p>We characterized truncations of the human vasopressin V2 receptor to determine the role of the intracellular C\u2010terminus (comprising about 44 amino acids) in receptor function and cell surface expression. In contrast to the wild\u2010type receptor, the naturally occurring mutant R337X failed to confer specific [<jats:sup>3<\/jats:sup>H]AVP binding to transfected cells. In addition, no vasopressin\u2010sensitive adenylyl cyclase was detectable in membrane preparations of these cells. Laser scanning microscopy revealed that c\u2010<jats:italic>myc<\/jats:italic> epitope\u2010 or green fluorescent protein\u2010tagged R337X mutant receptors were retained within the endoplasmic reticulum. Increasing the number of C\u2010terminal residues (truncations after codons 348, 354 and 356) restored G protein coupling, but revealed a length\u2010dependent reduction of cell surface expression. Replacement of positively charged residues within the C\u2010terminus by glutamine residues also decreased cell surface expression. A chimeric V2 receptor with the C\u2010terminus replaced by that of the \u03b2<jats:sub>2<\/jats:sub>\u2010adrenergic receptor did not bind [<jats:sup>3<\/jats:sup>H]AVP and was retained within the cell. These data suggest that residues in the N\u2010terminal part of the C\u2010terminus are necessary for correct folding and that C\u2010terminal residues are important for efficient cell surface expression.<\/jats:p>","DOI":"10.1016\/s0014-5793(98)00140-9","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T17:53:27Z","timestamp":1027619607000},"page":"57-62","source":"Crossref","is-referenced-by-count":60,"title":["Folding and cell surface expression of the vasopressin V2 receptor: requirement of the intracellular C\u2010terminus"],"prefix":"10.1002","volume":"424","author":[{"given":"Alexander","family":"Oksche","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcel","family":"Dehe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf","family":"Sch\u00fclein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Burkhard","family":"Wiesner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter","family":"Rosenthal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1998,3,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb05814.x"},{"key":"e_1_2_6_3_1","first-page":"1036","volume":"46","author":"Osawa S.","year":"1994","journal-title":"Mol. Endocrinol."},{"key":"e_1_2_6_4_1","first-page":"278","volume":"55","author":"Bichet D.G.","year":"1994","journal-title":"Am. J. Hum. Genet."},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/mend.11.6.9919"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3130611"},{"key":"e_1_2_6_7_1","first-page":"820","volume":"50","author":"Oksche A.","year":"1996","journal-title":"Mol. Pharmacol."},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.101.2.315"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)45341-5"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00878-7"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00532-X"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.31.19464"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)74280-X"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)45312-9"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.15.8772"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.52.1.21"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi970908a"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi962403a"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.15.8458"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.4.2279"},{"key":"e_1_2_6_21_1","unstructured":"Oksche A. and Rosenthal W. (1998) J. Mol. Med. (in press)."},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)38614-4"},{"key":"e_1_2_6_23_1","doi-asserted-by":"crossref","unstructured":"Tsukaguchi H. Matsubara H. Taketani S. Mori Y. Seido T. and Inada M. (1995) 96 2043\u20132050.","DOI":"10.1172\/JCI118252"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2FS0014-5793%2898%2900140-9","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2FS0014-5793(98)00140-9","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/S0014-5793%2898%2900140-9","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T07:09:04Z","timestamp":1694934544000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/S0014-5793%2898%2900140-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,3,6]]},"references-count":22,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[1998,3,6]]}},"alternative-id":["10.1016\/S0014-5793(98)00140-9"],"URL":"https:\/\/doi.org\/10.1016\/s0014-5793(98)00140-9","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,3,6]]}}}