{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:44:38Z","timestamp":1761540278789},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1998,10,7]],"date-time":"1998-10-07T00:00:00Z","timestamp":907718400000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1998,9,11]]},"abstract":"<jats:p>A recombinant GST\u2010Fyn\u2010SH2 domain was used to purify proteins from lysates of pervanadate treated EL4 cells. N\u2010terminal sequencing and molecular cloning of one of the purified polypeptides resulted in the identification of a novel adaptor protein that shares strong structural homology to the recently cloned Fyn\u2010associated adaptor protein SKAP55. This protein was termed SKAP\u2010HOM (<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/feb2s0014579398010400-math-si1.gif\" xlink:title=\"urn:x-wiley:00145793:media:feb2s0014579398010400:feb2s0014579398010400-math-si1\" \/>55 <jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/feb2s0014579398010400-math-si2.gif\" xlink:title=\"urn:x-wiley:00145793:media:feb2s0014579398010400:feb2s0014579398010400-math-si2\" \/>ologue). Despite their striking homology, SKAP55 and SKAP\u2010HOM have distinct characteristics. Thus, unlike SKAP55, which is exclusively expressed in T lymphocytes, SKAP\u2010HOM expression is ubiquitous. Furthermore, while SKAP55 is constitutively tyrosine phosphorylated in resting human T cells, SKAP\u2010HOM is expressed as a non\u2010phosphorylated protein in the absence of external stimulus but becomes phosphorylated following T cell activation. In addition, SKAP\u2010HOM does not associate with p59<jats:sup>fyn<\/jats:sup> in T cells although it represents a specific substrate for the kinase in COS cells. Finally, we demonstrate that, as previously shown for SKAP55, SKAP\u2010HOM interacts with the recently identified polypeptide SLAP\u2010130.<\/jats:p>","DOI":"10.1016\/s0014-5793(98)01040-0","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T12:19:54Z","timestamp":1027599594000},"page":"55-60","source":"Crossref","is-referenced-by-count":79,"title":["SKAP\u2010HOM, a novel adaptor protein homologous to the FYN\u2010associated protein SKAP55<sup>1<\/sup>"],"prefix":"10.1002","volume":"435","author":[{"given":"Anne","family":"Marie-Cardine","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne M.","family":"Verhagen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Eckerskorn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Burkhart","family":"Schraven","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1998,10,7]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.26.16077"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.14.7493"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.18.11674"},{"key":"e_1_2_5_5_1","unstructured":"Marie-Cardine A. 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