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Here, we examined whether the individual cysteine\u2010rich motifs of the CRD of PKD (referred to as cys1 and cys2) are functionally equivalent in mediating phorbol ester binding both in vivo and in vitro. Our results demonstrate that the cys1 and cys2 motifs of the CRD of PKD are functionally dissimilar, with the cys2 motif responsible for the majority of [<jats:sup>3<\/jats:sup>H]phorbol 12,13\u2010dibutyrate (PDB) binding, both in vivo and in vitro.<\/jats:p>","DOI":"10.1016\/s0014-5793(98)01189-2","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T15:15:33Z","timestamp":1027610133000},"page":"19-23","source":"Crossref","is-referenced-by-count":54,"title":["Dissimilar phorbol ester binding properties of the individual cysteine\u2010rich motifs of protein kinase D"],"prefix":"10.1002","volume":"437","author":[{"given":"Teresa","family":"Iglesias","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharon","family":"Matthews","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrique","family":"Rozengurt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1998,12,2]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.9.7.7737456"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pa.36.040196.002405"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0959-440X(95)80103-0"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.48.28495"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560060228"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.13.4868"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)55274-1"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1763327"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)32119-1"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)42034-5"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.37.21852"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90011-X"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.2940120308"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.9.4627"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.31.18299"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.18.8572"},{"key":"e_1_2_4_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(95)00141-7"},{"key":"e_1_2_4_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.3.1455"},{"key":"e_1_2_4_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb01012.x"},{"key":"e_1_2_4_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)37580-4"},{"key":"e_1_2_4_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(96)00116-0"},{"key":"e_1_2_4_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb05848.x"},{"key":"e_1_2_4_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.1.410"},{"key":"e_1_2_4_25_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0250565"},{"key":"e_1_2_4_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.32.20245"},{"key":"e_1_2_4_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041180205"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2FS0014-5793%2898%2901189-2","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2FS0014-5793(98)01189-2","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/S0014-5793%2898%2901189-2","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T19:46:29Z","timestamp":1694893589000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/S0014-5793%2898%2901189-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,10,16]]},"references-count":26,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[1998,10,16]]}},"alternative-id":["10.1016\/S0014-5793(98)01189-2"],"URL":"https:\/\/doi.org\/10.1016\/s0014-5793(98)01189-2","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,10,16]]}}}