{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T12:52:32Z","timestamp":1768481552572,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1999,8,30]],"date-time":"1999-08-30T00:00:00Z","timestamp":935971200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1999,8,27]]},"abstract":"<jats:p>Folding of the green fluorescent protein (GFP) from <jats:italic>Aequorea victoria<\/jats:italic> is characterized by autocatalytic formation of its p\u2010hydroxybenzylideneimidazolidone chromophore, which is located in the center of an 11\u2010stranded \u03b2\u2010barrel. We have analyzed the in vivo folding of 20 circularly permuted variants of GFP and find a relatively low tolerance towards disruption of the polypeptide chain by introduction of new termini. All permuted variants with termini in strands of the \u03b2\u2010barrel and about half of the variants with termini in loops lost the ability to form the chromophore. The thermal stability of the permuted GFPs with intact chromophore is very similar to that of the wild\u2010type, indicating that chromophore\u2010side chain interactions strongly contribute to the extraordinary stability of GFP.<\/jats:p>","DOI":"10.1016\/s0014-5793(99)01044-3","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T19:16:26Z","timestamp":1027624586000},"page":"283-289","source":"Crossref","is-referenced-by-count":122,"title":["Circularly permuted variants of the green fluorescent protein"],"prefix":"10.1002","volume":"457","author":[{"given":"Simon","family":"Topell","sequence":"first","affiliation":[]},{"given":"Jens","family":"Hennecke","sequence":"additional","affiliation":[]},{"given":"Rudi","family":"Glockshuber","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1999,8,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(83)80344-1"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(83)80265-4"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2643160"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.2531"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.21.11591"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5280.1392"},{"key":"e_1_2_6_8_1","unstructured":"Wu C.-K. Liu Z.-J. Rose J.P. Inouye S. Tsuji F. Tsien R.Y. Remington S.J. and Wang B.C. (1997) in: Bioluminescence and Chemiluminescence (Hastings J. Kricka L.J. and Stanley P.E. Eds.) pp. 399\u2013402 Wiley Chichester."},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1096-1246"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00056a003"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(00)89099-4"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.24.13617"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.26.12501"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(92)90691-H"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.23.13549"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(81)91599-0"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00262a003"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(98)77870-1"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.12.6803"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/42264"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1097-961"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0396-315"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(97)78307-3"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)74500-1"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(85)90120-9"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1980.tb03755.x"},{"key":"e_1_2_6_27_1","unstructured":"Ward W.W. (1981) in: Bioluminescence and Chemiluminescence: Basic Chemistry and Analytical Applications (DeLuca M. and McElroy D.W. Eds.) pp. 235\u2013242 Academic Press New York."},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(97)80153-4"},{"key":"e_1_2_6_29_1","unstructured":"Clontech Laboratories (1997) Living Colors User Manual PT2040-1 p. 12."},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.16.8362"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0597-361"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0104(96)00340-0"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi970281w"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00208801"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.45.28545"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00692-3"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.2.623"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00732-2"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0263-7855(96)00009-4"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2FS0014-5793%2899%2901044-3","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2FS0014-5793(99)01044-3","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/S0014-5793%2899%2901044-3","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T07:57:38Z","timestamp":1694937458000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/S0014-5793%2899%2901044-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,8,27]]},"references-count":38,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1999,8,27]]}},"alternative-id":["10.1016\/S0014-5793(99)01044-3"],"URL":"https:\/\/doi.org\/10.1016\/s0014-5793(99)01044-3","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,8,27]]}}}