{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T17:43:12Z","timestamp":1773682992410,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1999,9,10]],"date-time":"1999-09-10T00:00:00Z","timestamp":936921600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1999,9,17]]},"abstract":"<jats:p>The heat shock protein (Hsp) HtpG is a member of the Hsp90 protein family. We cloned a single\u2010copy gene encoding a homologue of HtpG from the unicellular cyanobacterium<jats:italic>Synechococcus<\/jats:italic>sp. PCC 7942. Sequence alignment with HtpGs from other prokaryotes revealed unique features in the cyanobacterial HtpG primary sequence. A monocistronic mRNA of the<jats:italic>htpG<\/jats:italic>gene increased transiently in response to heat shock. In order to elucidate the role of HtpG in vivo, we inactivated the<jats:italic>htpG<\/jats:italic>gene by targeted mutagenesis. Although the mutation did not affect the photoautotrophic growth at 30 and 42\u00b0C, the mutant cells were unable to grow at 45\u00b0C. They lost both basal and acquired thermotolerances. These results indicate that HtpG plays an essential role for the thermal stress management in cyanobacteria, the first such an example for either a photosynthetic or a prokaryotic organism.<\/jats:p>","DOI":"10.1016\/s0014-5793(99)01134-5","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T17:52:28Z","timestamp":1027619548000},"page":"117-123","source":"Crossref","is-referenced-by-count":78,"title":["HtpG is essential for the thermal stress management in cyanobacteria"],"prefix":"10.1002","volume":"458","author":[{"given":"Naoki","family":"Tanaka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Nakamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1999,9,10]]},"reference":[{"key":"e_1_2_5_2_1","unstructured":"Parcell D.A. and Lindquist S. (1994) in: The Biology of Heat Shock Proteins and Molecular Chaperones (Morimoto R.I. Tissieres A. and Georgopoulos C. Eds.) pp. 457\u2013494 Cold Spring Harbor Laboratory Press New York."},{"key":"e_1_2_5_3_1","doi-asserted-by":"crossref","unstructured":"Scheibel T. and Buchner J. (1997) in: Guidebook to Molecular Chaperones and Protein-Folding Catalysts (Gething M.-J. Ed.) pp. 147\u2013151 Oxford University Press Oxford.","DOI":"10.1093\/oso\/9780198599494.003.0057"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(99)01373-0"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/358169a0"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.13.7288"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00660.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.5.2641"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)83755-3"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.24.14412"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.7.2977-2983.1988"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(96)00317-4"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004380051004"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.9.9.3919"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.161.3.1125-1130.1985"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.9.5079-5088.1990"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00034959"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4838(96)00037-4"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00380.x"},{"key":"e_1_2_5_20_1","first-page":"1","volume":"111","author":"Rippka R.","year":"1979","journal-title":"J. Gen. Microbiol."},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.11.2998-3003.1995"},{"key":"e_1_2_5_22_1","unstructured":"Sambrook J. Fitsch E.F. and Maniatis T. (1989) Molecular Cloning: a Laboratory Manual 2nd edn. Cold Spring Harbor Laboratory Press New York."},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.pcp.a029479"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)53055-5"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/3.3.109"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80203-2"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80314-1"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.38.23843"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(84)90059-3"},{"key":"e_1_2_5_30_1","doi-asserted-by":"crossref","unstructured":"Sidler W.A. (1994) in: The Molecular Biology of Cyanobacteria (Bryant D.A. Ed.) pp. 139\u2013216 Kluwer.","DOI":"10.1007\/978-94-011-0227-8_7"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)52401-7"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.19.5165-5172.1998"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2FS0014-5793%2899%2901134-5","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2FS0014-5793(99)01134-5","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/S0014-5793%2899%2901134-5","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,12,31]],"date-time":"2023-12-31T23:34:57Z","timestamp":1704065697000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/S0014-5793%2899%2901134-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,9,10]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1999,9,17]]}},"alternative-id":["10.1016\/S0014-5793(99)01134-5"],"URL":"https:\/\/doi.org\/10.1016\/s0014-5793(99)01134-5","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,9,10]]}}}