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Most (yet not all) amino acid replacements at both positions resulted in severe reduction of the fMet\u2010tRNA binding activity of IF2 C without grossly altering its structure. Our work demonstrates that: (a) both Cys residues are buried within an hydrophobic core and not accessible to protonation or chemical substitution, (b) neither Cys is functionally essential and (c) both Cys residues are located near the active site, probably without participating directly in fMet\u2010tRNA binding.<\/jats:p>","DOI":"10.1016\/s0014-5793(99)01280-6","type":"journal-article","created":{"date-parts":[[2002,10,11]],"date-time":"2002-10-11T10:59:48Z","timestamp":1034333988000},"page":"332-336","source":"Crossref","is-referenced-by-count":9,"title":["The fMet\u2010tRNA binding domain of translational initiation factor IF2: role and environment of its two Cys residues"],"prefix":"10.1002","volume":"459","author":[{"given":"Rolf","family":"Misselwitz","sequence":"first","affiliation":[]},{"given":"Karin","family":"Welfle","sequence":"additional","affiliation":[]},{"given":"Christoph","family":"Krafft","sequence":"additional","affiliation":[]},{"given":"Heinz","family":"Welfle","sequence":"additional","affiliation":[]},{"given":"Letizia","family":"Brandi","sequence":"additional","affiliation":[]},{"given":"Enrico","family":"Caserta","sequence":"additional","affiliation":[]},{"given":"Claudio O","family":"Gualerzi","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1999,10,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00477a001"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0116"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00549a020"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1986.tb04598.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)55305-9"},{"key":"e_1_2_5_7_1","doi-asserted-by":"crossref","unstructured":"Spurio R. 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