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Neither antigen receptor (Ig) crosslinking nor stimulation of B\u2010cells with phorbol ester or H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> affected Btk\/PKC\u03bc interaction. GST precipitation analysis revealed association of the Btk pleckstrin\/Tec homology domain with PKC\u03bc. Transient overexpression of PKC\u03bc deletion mutants as well as expression of selected PKC\u03bc domains in 293T cells revealed that both the kinase domain and the regulatory C1 region are independently capable of binding to the Btk PH\u2010TH domain. These data show the existence of a PKC\u03bc\/Btk complex in vivo and identify two PKC\u03bc domains that participate in Btk interaction.<\/jats:p>","DOI":"10.1016\/s0014-5793(99)01424-6","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T17:52:28Z","timestamp":1027619548000},"page":"68-72","source":"Crossref","is-referenced-by-count":35,"title":["Bruton's tyrosine kinase (Btk) associates with protein kinase C 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