{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T07:25:10Z","timestamp":1762673110424},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1999,11,15]],"date-time":"1999-11-15T00:00:00Z","timestamp":942624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["FEBS Letters"],"published-print":{"date-parts":[[1999,11,19]]},"abstract":"<jats:p>Each amino acid in putative transmembrane helix VI and its flanking regions, from Ser\u2010156 to Thr\u2010185, of a Cys\u2010free mutant of the Tn10\u2010encoded metal\u2010tetracycline\/H<jats:sup>+<\/jats:sup> antiporter (TetA(B)) was individually replaced by Cys. All of the cysteine\u2010scanning mutants showed a normal level of tetracycline resistance except for the S156C mutant, which showed moderate resistance, indicating that there is no essential residue located in this region. All 20 mutants from S159C to W178C showed no reactivity with <jats:italic>N<\/jats:italic>\u2010ethylmaleimide (NEM), whereas the mutants of the flanking regions from S156C to H158C and F179C to T185C were highly or moderately reactive with NEM. These results indicate that like transmembrane helices III and IX, the transmembrane helix VI comprising residues Ser\u2010159\u2013Trp\u2010178 is totally embedded in the hydrophobic environment.<\/jats:p>","DOI":"10.1016\/s0014-5793(99)01490-8","type":"journal-article","created":{"date-parts":[[2002,7,25]],"date-time":"2002-07-25T17:52:28Z","timestamp":1027619548000},"page":"315-318","source":"Crossref","is-referenced-by-count":20,"title":["Cysteine\u2010scanning mutagenesis around transmembrane segment VI of Tn10\u2010encoded metal\u2010tetracycline\/H<sup>+<\/sup> antiporter"],"prefix":"10.1002","volume":"461","author":[{"given":"Satoko","family":"Konishi","sequence":"first","affiliation":[]},{"given":"Shinobu","family":"Iwaki","sequence":"additional","affiliation":[]},{"given":"Tomomi","family":"Kimura-Someya","sequence":"additional","affiliation":[]},{"given":"Akihito","family":"Yamaguchi","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[1999,11,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.7.3974"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)34044-X"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.36.4.695"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.1.580"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi961568g"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.9.5243"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.35.22545"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.27.19480"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.49.32806"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a124445"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(97)00088-1"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.2.488"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00001a002"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)55428-4"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)80872-G"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(78)90149-6"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)42544-6"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.12.13.1281"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.13.5973"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560040319"}],"container-title":["FEBS Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2FS0014-5793%2899%2901490-8","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1016%2FS0014-5793(99)01490-8","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1016\/S0014-5793%2899%2901490-8","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T19:52:53Z","timestamp":1694893973000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1016\/S0014-5793%2899%2901490-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,11,15]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1999,11,19]]}},"alternative-id":["10.1016\/S0014-5793(99)01490-8"],"URL":"https:\/\/doi.org\/10.1016\/s0014-5793(99)01490-8","archive":["Portico"],"relation":{},"ISSN":["0014-5793","1873-3468"],"issn-type":[{"value":"0014-5793","type":"print"},{"value":"1873-3468","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,11,15]]}}}